{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:50:39Z","timestamp":1787784639216,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T00:00:00Z","timestamp":1418169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Centre of Experimental and Clinical Respirology I"},{"name":"Centre of Experimental and Clinical Respirology II"},{"name":"BioMed"},{"DOI":"10.13039\/501100006109","name":"VEGA","doi-asserted-by":"crossref","award":["1\/0127\/13"],"award-info":[{"award-number":["1\/0127\/13"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This experimental <jats:italic>in vitro<\/jats:italic> study examined differences in the expression and activity of calcium release\u2010activated calcium (<jats:styled-content style=\"fixed-case\">CRAC<\/jats:styled-content>) channels of human term\u2010pregnant and non\u2010pregnant myometrium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The tissue samples were obtained from term\u2010pregnant myometrium in labor of women undergoing cesarean section and from non\u2010pregnant myometrium of women undergoing total hysterectomy due to uterine myoma. The expression of <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>rai1 protein, a pore\u2010forming subunit of <jats:styled-content style=\"fixed-case\">CRAC<\/jats:styled-content> channels, in human myometrium was examined using immunohistochemistry. <jats:styled-content style=\"fixed-case\">CRAC<\/jats:styled-content> channel involvement in the amplitude and frequency of myometrial contractions was evaluated <jats:italic>in vitro<\/jats:italic> using a tissue bath method with a <jats:styled-content style=\"fixed-case\">CRAC<\/jats:styled-content> ion channel blocker 3\u2010fluropyridine\u20104\u2010carboxylic acid (<jats:styled-content style=\"fixed-case\">FPCA<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Decreased <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>rai1 expression was observed in human term\u2010pregnant laboring myometrium compared with non\u2010pregnant myometrium. However, the initial oxytocin\u2010induced contraction of myometrium was significantly suppressed at different doses of <jats:styled-content style=\"fixed-case\">FPCA<\/jats:styled-content> in both non\u2010pregnant human isolated myometrium and non\u2010pregnant myometrium. The frequency of contractions was the most significantly reduced at the lowest dose of <jats:styled-content style=\"fixed-case\">FPCA<\/jats:styled-content> in non\u2010pregnant myometrium and remained suppressed at all doses of <jats:styled-content style=\"fixed-case\">FPCA<\/jats:styled-content> in term\u2010pregnant myometrium. Salbutamol was shown as more effective in suppression of amplitude in term\u2010pregnant isolated myometrium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results provide the first information about the changes in the <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>rai1 protein expression and activity of human myometrial <jats:styled-content style=\"fixed-case\">CRAC<\/jats:styled-content> channels in term\u2010pregnant laboring myometrium.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12626","type":"journal-article","created":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T10:54:09Z","timestamp":1418208849000},"page":"704-711","source":"Crossref","is-referenced-by-count":4,"title":["Orai1 protein expression and the role of calcium release\u2010activated calcium channels in the contraction of human term\u2010pregnant and non\u2010pregnant myometrium"],"prefix":"10.1111","volume":"41","author":[{"given":"Martina","family":"Sutovska","sequence":"first","affiliation":[{"name":"Department of Pharmacology Jessenius Faculty of Medicine Martin Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Kocmalova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Jessenius Faculty of Medicine Martin Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimira","family":"Sadlonova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Jessenius Faculty of Medicine Martin Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karol","family":"Dokus","sequence":"additional","affiliation":[{"name":"Clinic of Obstetrics and Gynecology Jessenius Faculty of Medicine and Martin University Hospital Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Adamkov","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology Jessenius Faculty of Medicine Martin Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Luptak","sequence":"additional","affiliation":[{"name":"Clinic of Urology Jessenius Faculty of Medicine and Martin University Hospital Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sona","family":"Franova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Jessenius Faculty of Medicine Martin Comenius University Bratislava  Martin Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0777-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/eph8602114"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2009.02.010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-010-0383-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/355353a0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.901"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00057.2003"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3136"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-008-0533-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-2888-2_16"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00820.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465\u20109921\u20107\u2010119"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.091082"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.233353"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00169"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2010.01340.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-2257"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.4.2044"},{"key":"e_1_2_7_21_1","unstructured":"WhittenJP PeiJ CaoJet\u2009al.Compounds that modulate intracellular calcium.2011. 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