{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T04:12:11Z","timestamp":1695528731313},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T00:00:00Z","timestamp":1418169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To determine whether <jats:italic>octamer\u2010binding transcription factor 4<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">OCT<\/jats:styled-content>\u20104<\/jats:italic>) and <jats:italic>deleted in azoospermia like<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">DAZL<\/jats:styled-content><\/jats:italic>) are expressed among cells with human papilloma virus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) infection and cervical intraepithelial neoplasia (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>) lesions and quantify their relative expression when compared with normal cervical cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cervical cells derived from normal cell cultures, <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> lesions and <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> lesions were cultured in Dulbecco's modified Eagle's medium supplemented with 20% amniotic fluid and 5\u2009ng\/mL basic fibroblast growth factor at 37\u00b0C and humidified 10% <jats:styled-content style=\"fixed-case\">CO<jats:sub>2<\/jats:sub><\/jats:styled-content> in air. Real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) was carried out using <jats:italic><jats:styled-content style=\"fixed-case\">G6PD<\/jats:styled-content><\/jats:italic> as a reference. We used <jats:styled-content style=\"fixed-case\">REST<\/jats:styled-content> for statistical analysis of real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Whereas <jats:italic><jats:styled-content style=\"fixed-case\">DAZL<\/jats:styled-content><\/jats:italic> was not expressed either in normal cultures or <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> lesions, <jats:italic><jats:styled-content style=\"fixed-case\">OCT<\/jats:styled-content>\u20104<\/jats:italic> was expressed in all examined cell lines. Moreover its relative expression was significantly upregulated among cultures of <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>\u2010infected cells (<jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content>, 11.003; 95%<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 0.054\u201336\u2009704.527, <jats:italic>P<\/jats:italic>\u2009=\u20090.042), an observation that was also close to statistical significance among cultures of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content> lesions (<jats:italic>P<\/jats:italic>\u2009=\u20090.066).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The relative expression of <jats:italic><jats:styled-content style=\"fixed-case\">OCT<\/jats:styled-content>\u20104<\/jats:italic> is upregulated during the early, preinvasive stages of cervical carcinogenesis. Future studies should investigate its potential as a screening marker and as a possible target of therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12640","type":"journal-article","created":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T10:56:27Z","timestamp":1418208987000},"page":"763-767","source":"Crossref","is-referenced-by-count":5,"title":["<i><scp>OCT<\/scp>\u20104<\/i> and <i><scp>DAZL<\/scp><\/i> expression in precancerous lesions of the human uterine cervix"],"prefix":"10.1111","volume":"41","author":[{"given":"Konstantinos","family":"Stefanidis","sequence":"first","affiliation":[{"name":"Laboratory of Stem Cells Division of Reproductive Medicine Alexandra Maternity Hospital  Athens Greece"},{"name":"1st Department of Obstetrics and Gynecology Medical School University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasileios","family":"Pergialiotis","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cells Division of Reproductive Medicine Alexandra Maternity Hospital  Athens Greece"},{"name":"1st Department of Obstetrics and Gynecology Medical School University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Christakis","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cells Division of Reproductive Medicine Alexandra Maternity Hospital  Athens Greece"},{"name":"1st Department of Obstetrics and Gynecology Medical School University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Patta","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cells Division of Reproductive Medicine Alexandra Maternity Hospital  Athens Greece"},{"name":"1st Department of Obstetrics and Gynecology Medical School University of Athens  Athens 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