{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T14:51:44Z","timestamp":1758811904857,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T00:00:00Z","timestamp":1432512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["30973206, 81170594"],"award-info":[{"award-number":["30973206, 81170594"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Distinguished Young Scholars Foundation","award":["30325011"],"award-info":[{"award-number":["30325011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Aquaporins (AQP) are water channel proteins, and some play an important role in maternal\u2013fetal fluid exchange. The present study aimed to measure the osmotic water permeability in primary cultures of trophoblast cells from AQP1\u2010deficient (AQP1<jats:sup>\u2013\/\u2013<\/jats:sup>) pregnant mice and to define the quantitative role of AQP1 in water transport across the trophoblast plasma membrane.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Trophoblast cells were obtained from placental tissue cell culture of AQP1<jats:sup>\u2013\/\u2013<\/jats:sup> pregnant mice and were characterized by cytokeratin 7 immunostaining. The expression of the AQP1 gene in trophoblast cells of wild\u2010type (AQP1<jats:sup>+\/+<\/jats:sup>) mice was confirmed by immunofluorescence. The osmotic water permeability of trophoblast plasma membranes was measured by a calcein fluorescence quenching method in response to osmotic gradients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A primary cell culture system for trophoblasts was successfully established. Immunofluorescence showed the expression of AQP1 in the trophoblast cell membrane of AQP1<jats:sup>+\/+<\/jats:sup> mice. The osmotic water permeability of AQP1<jats:sup>\u2013\/\u2013<\/jats:sup> trophoblast cells was significantly lower than that in AQP1<jats:sup>+\/+<\/jats:sup> trophoblast cells, in response to both hypotonic and hypertonic challenges.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results suggest an important role of AQP1\u2010mediated plasma membrane water permeability in maternal\u2013fetal fluid balance and also provide a potential direction for the identification of therapeutic targets for the treatment of abnormalities in amniotic fluid volume.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12737","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T23:58:28Z","timestamp":1432598308000},"page":"1399-1405","source":"Crossref","is-referenced-by-count":6,"title":["Osmotic water permeability diversification in primary trophoblast cultures from aquaporin 1\u2010deficient pregnant mice"],"prefix":"10.1111","volume":"41","author":[{"given":"Xiao\u2010Yan","family":"Sha","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Guangzhou Women and Children's Medical Centre Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Shu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Guangzhou Women and Children's Medical Centre Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong\u2010Hui","family":"Ma","sequence":"additional","affiliation":[{"name":"Central Research Laboratory Jilin University Bethune Second Hospital  Changchun 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