{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:10:12Z","timestamp":1790611812846,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To report the outcome of intracytoplasmic sperm injection (ICSI) cycles using fresh or cryopreserved\u2010thawed testicular spermatozoa of men with Klinefelter syndrome (KS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Medical records of 83 azoospermic men with KS who underwent testicular sperm extraction (TESE) were reviewed. The clinical parameters for predicting sperm retrieval and fertilization, implantation, pregnancy and live birth rates of ICSI cycles in these patients were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 88 TESE procedures were performed with sperm retrieval rates of 39.8% per cycle (35\/88) and 42.1% per patient (35\/83). None of the studied clinical parameters were found to be informative in predicting successful sperm recovery. A total of 41 embryo transfer cycles were carried out using fresh testicular spermatozoa in 30, cryopreserved\u2010thawed spermatozoa in 10 and cryopreserved\u2010thawed embryo replacement in one. The fertilization and clinical pregnancy rates were comparable at 52.7% and 51.6% with fresh and 48.3% and 60% with cryopreserved\u2010thawed testicular spermatozoa groups, respectively. Twenty\u2010two clinical pregnancies were obtained, including 14 singletons, five twins, two triplets and one quadruplet and ended with the delivery of 13 singletons and six twins. In total, out of 25 delivered fetuses, four died (3 female, 1 male) following delivery and 21 newborns (14 female, 7 male) were healthy with a female to male ratio of 2:1. Conclusions<\/jats:p><jats:p>We concluded that no clinical or laboratory parameter predicts the presence of spermatozoa in patients with KS, except the TESE procedure itself. The use of fresh or cryopreserved\u2010thawed spermatozoa on ICSI cycle outcomes are equally successful in patients with KS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13090","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T07:25:59Z","timestamp":1477553159000},"page":"1558-1566","source":"Crossref","is-referenced-by-count":30,"title":["Outcome of intracytoplasmic sperm injection using fresh and cryopreserved\u2010thawed test\u0131cular spermatozoa in 83 azoospermic men with Klinefelter syndrome"],"prefix":"10.1111","volume":"42","author":[{"given":"Kubilay","family":"Vicdan","sequence":"first","affiliation":[{"name":"Ankara Private IVF Center  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cem","family":"Akarsu","sequence":"additional","affiliation":[{"name":"Ankara Private IVF Center  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eran","family":"S\u00f6zen","sequence":"additional","affiliation":[{"name":"Ankara Private IVF Center  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcu","family":"Bulu\u00e7","sequence":"additional","affiliation":[{"name":"Ankara Private IVF Center  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arzu","family":"Vicdan","sequence":"additional","affiliation":[{"name":"Department of Genetics Ankara University Medical School  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y\u0131ld\u0131r\u0131m","family":"Y\u0131lmaz","sequence":"additional","affiliation":[{"name":"Ankara Private IVF Center  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kutay","family":"Bibero\u011flu","sequence":"additional","affiliation":[{"name":"Department of Obcstetric and Gynecology Gazi University Medical School  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01213097"},{"key":"e_1_2_6_3_1","first-page":"93","article-title":"Chromosome abnormalities and male infertility","volume":"6","author":"Yoshida A","year":"1996","journal-title":"Assist Rep Rev"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)00539-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55334-5"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019462"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.11.2447"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/16.12.2616"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(03)01157-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2004.07.013"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-2322"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2006.05.028"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.01.005"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.03.056"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-006-9079-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.05.019"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60023-5"},{"key":"e_1_2_6_18_1","first-page":"424","article-title":"Births using frozen\u2010thawed spermatozoa and frozen\u2010thawed embryos in two azoospermic patients with nonmosaic Klinefelter's syndrome","volume":"8","author":"Vicdan K","year":"2007","journal-title":"J Turkish\u2010German Gynecol Assoc"},{"key":"e_1_2_6_19_1","volume-title":"WHO Laboratory Manual for the Examination and Processing of Human Semen","author":"World Health Organization","year":"2010"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.06.011"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)01556-9"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(02)03092-3"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.4.886"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(03)00403-5"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh253"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.05.053"},{"key":"e_1_2_6_27_1","first-page":"1508","article-title":"Four pregnancies in non\u2010mosaic Klinefelter's syndrome using cryopreserved\u2010thawed testicular spermatozoa","volume":"5","author":"Okada H","year":"2005","journal-title":"Fertil Steril"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/14.1.131"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep431"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2013.09.021"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2014.00231.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.09.038"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-3083"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2012.11203.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65411-0"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.3.653"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2004.12.059"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64575-2"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2013.00148.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.07.1136"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.8.1804"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13090","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:39:20Z","timestamp":1695573560000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.13090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,27]]},"references-count":40,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/jog.13090"],"URL":"https:\/\/doi.org\/10.1111\/jog.13090","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,27]]}}}