{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T03:14:49Z","timestamp":1761621289378},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T00:00:00Z","timestamp":1476662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to develop a novel optical imaging system for detecting protoporphyrin IX (PpIX) autofluorescence, to prove that PpIX autofluorescence is as useful as 5\u2010aminolevulinic acid (5\u2010ALA)\u2010induced fluorescence for detecting and localizing cervical cancer, and to monitor the change in PpIX autofluorescence or induced PpIX fluorescence before, during, and after photodynamic therapy (PDT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>TC\u20101 cells \u2013 highly tumorigenic cells immortalized using human papillomavirus type 16 proteins E6 and E7 \u2013 were subcutaneously grafted into the thighs of nude mice. The suspected tumor tissues were visualized using autofluorescence imaging and induced fluorescence imaging under 5\u2010ALA administration. When the 5\u2010ALA\u2010induced PpIX was sufficiently accumulated in tumor tissues, PDT was performed using a 635\u2010nm laser. We observed the change in fluorescence intensity during PDT. For 3 weeks after PDT, we monitored tumor remission by using white\u2010light imaging and fluorescence imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The transplanted cells were visualized by PpIX autofluorescence, which was induced by heme synthesis. After 5\u2010ALA administration, PpIX could be targeted by using PDT, which decreased PpIX autofluorescence. Photobleaching is useful for monitoring PDT dosimetry and for determining the photodynamic response to therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PpIX autofluorescence clearly differentiated the tumor from adjacent normal tissues. The results of PpIX autofluorescence imaging and 5\u2010ALA\u2010induced fluorescence imaging were identical. PpIX autofluorescence imaging is a simple and cost\u2010effective cervical cancer screening method that could be performed during or after PDT to ensure effective treatment or remission as a change in fluorescence intensity can be observed in real time without a blinding effect.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13101","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T09:12:58Z","timestamp":1476695578000},"page":"1846-1853","source":"Crossref","is-referenced-by-count":7,"title":["Multispectral autofluorescence imaging for detection of cervical lesions: A preclinical study"],"prefix":"10.1111","volume":"42","author":[{"given":"Soo\u2010Jin","family":"Bae","sequence":"first","affiliation":[{"name":"Korea Electrotechnology Research Institute  Seoul Korea"},{"name":"Department of Obstetrics and Gynecology Catholic Research Institutes of Medical Science, The Catholic University of Korea  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae\u2010Sic","family":"Lee","sequence":"additional","affiliation":[{"name":"Korea Electrotechnology Research Institute  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Berezin","sequence":"additional","affiliation":[{"name":"Korea Electrotechnology Research Institute  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uk","family":"Kang","sequence":"additional","affiliation":[{"name":"Korea Electrotechnology Research Institute  Seoul Korea"},{"name":"SNUH Biomedical Research Institute  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keun\u2010Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Catholic Research Institutes of Medical Science, The Catholic University of Korea  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20138"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/jwh.2011.3385"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.52.6.342"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji187"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-5-1139"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8019.2005.05023.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(95)07265-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/clm.1996.14.59"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s101030050049"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1999.tb05307.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1443-1661.2003.00239.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-011-0462-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1238\/Physica.Topical.110a00039"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2010.11.029"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01222-5"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.48.3.314"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(92)85108-7"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocn.2012.03.013"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-8888com"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13101","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T20:17:57Z","timestamp":1695500277000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.13101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,17]]},"references-count":19,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/jog.13101"],"URL":"https:\/\/doi.org\/10.1111\/jog.13101","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,17]]}}}