{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T23:12:49Z","timestamp":1789945969668,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T00:00:00Z","timestamp":1516665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22591198"],"award-info":[{"award-number":["22591198"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Antenatal maternal administration of magnesium sulfate (MgSO<jats:sub>4<\/jats:sub>) reduces cerebral palsy in preterm infants. However, it remains controversial as to whether it also reduces occurrence of white matter damage, or periventricular leukomalacia. We assessed the effect of MgSO<jats:sub>4<\/jats:sub> against white matter damage induced by hypoxic\u2010ischemic insult using a neonatal rat model and culture of premyelinating oligodendrocytes (pre\u2010OL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rat pups at postnatal day (P) 6 were administered either MgSO<jats:sub>4<\/jats:sub> or vehicle intraperitoneally before hypoxic\u2010ischemic insult (unilateral ligation of the carotid artery followed by 6% oxygen for 1\u2009h). The population of oligodendrocyte (OL) markers and CD\u201068\u2010positive microglia at P11, and TdT\u2010mediated biotin\u201016\u2010dUTP nick\u2010end labeling (TUNEL)\u2010positive cells at P8 were evaluated in pericallosal white matter. Primary cultures of mouse pre\u2010OL were subjected to oxygen glucose deprivation condition, and the lactate dehydrogenase release from culture cells was evaluated to assess cell viability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pretreatment with MgSO<jats:sub>4<\/jats:sub> attenuated the loss of OL markers, such as myelin basic protein and Olig2, in ipsilateral pericallosal white matter and decreased the number of CD\u201068\u2010positive microglia and TUNEL\u2010positive cells <jats:italic>in vivo<\/jats:italic>. Pretreatment with MgSO<jats:sub>4<\/jats:sub> also inhibited lactate dehydrogenase release from pre\u2010OL induced by oxygen glucose deprivation <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Pretreatment with MgSO<jats:sub>4<\/jats:sub> attenuates white matter damage by preventing cell death of pre\u2010OL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13568","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T03:58:43Z","timestamp":1516766323000},"page":"601-607","source":"Crossref","is-referenced-by-count":21,"title":["Pretreatment with magnesium sulfate attenuates white matter damage by preventing cell death of developing oligodendrocytes"],"prefix":"10.1111","volume":"44","author":[{"given":"Takahiro","family":"Seyama","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The University of Tokyo Hospital  Tokyo 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