{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:19:23Z","timestamp":1790313563670,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T00:00:00Z","timestamp":1533772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472429"],"award-info":[{"award-number":["81472429"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The goal of this study was to analyze the specificity of p16\/Ki67 dual staining in the detection of high\u2010grade cervical lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 223 patients with an average age of 39\u2009years old were enrolled in this study. All samples were analyzed by p16\/Ki67 immunocytochemical dual staining, liquid\u2010based cytology and high\u2010risk human papillomavirus (HR\u2010HPV) test. Diagnosis of each patient was verified by histopathological test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The specificity of p16\/Ki67 dual staining was 68.33%, which was significantly higher than that of cytology (38.33%) and HR\u2010HPV test (21.67%) (<jats:italic>P<\/jats:italic> &lt;\u20090.05) for CIN2+ detection. p16\/Ki67 dual staining had similar sensitivity with HR\u2010HPV test for CIN2+ detection (90.18% vs 93.87%, <jats:italic>P<\/jats:italic> =\u20090.286). In atypical squamous cells of undetermined significance (ASC\u2010US) and low\u2010grade squamous intraepithelial lesion (LSIL) cases, the specificity of p16\/Ki67 dual staining was significantly higher than that of HPV test (66.67% vs 3.70%, <jats:italic>P<\/jats:italic> &lt;\u20090.05) and its sensitivity was similar to that of HPV test for CIN2+ detection. The sensitivity and specificity of dual staining for CIN2+ detection in HR\u2010HPV positive women were 90.85% and 70.21%, respectively, which were higher than those of cytology (83.01% and 42.55%) and HPV16\/18 test (70.59% and 44.68%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>p16\/Ki67 dual staining could improve the specificity of high\u2010grade cervical lesions detection and have similar sensitivity to HPV test for CIN2+ detection. When triaging women with ASC\u2010US or LSIL liquid\u2010based cytology, compared with positive HR\u2010HPV, the specificity of CIN2+ lesion detection was increased by p16\/Ki67 dual staining. p16\/Ki67 dual staining could reduce colposcopy referrals and avoid excessive diagnosis and treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13760","type":"journal-article","created":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T05:39:59Z","timestamp":1533879599000},"page":"2077-2084","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["p16\/Ki67 dual staining improves the detection specificity of high\u2010grade cervical lesions"],"prefix":"10.1111","volume":"44","author":[{"given":"Ruiyi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefei","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"You","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21262"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000001708"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(11)70145-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.12788\/jcso.0039"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)06809-X"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26017"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21233"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20140"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21511"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.80"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1001\/jama.1989.03430070079034","article-title":"The 1988 Bethesda System for reporting cervical\/vaginal cytological diagnoses. National Cancer Institute Workshop","volume":"262","year":"1989","journal-title":"JAMA"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2120"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-0776"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2007\/678793"},{"key":"e_1_2_7_16_1","first-page":"669","article-title":"Both cell proliferation and apoptosis increase with lesion grade in cervical neoplasia but do not correlate with human papillomavirus type","volume":"56","author":"Isacson C","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.3.5.837"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1586\/epr.12.13"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0b013e31826916c7"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31827001d5"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.34"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt235"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.07.047"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2008.00623.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21542"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134445"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-014-0688-0"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPZ5JY6FCVNMOT"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2016.10.031"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv257"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T19:50:05Z","timestamp":1694893805000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.13760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,9]]},"references-count":29,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/jog.13760"],"URL":"https:\/\/doi.org\/10.1111\/jog.13760","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,9]]},"assertion":[{"value":"2018-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}