{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T19:11:01Z","timestamp":1777057861003,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T00:00:00Z","timestamp":1553472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key R&D Program of China","award":["2016YFC1303100"],"award-info":[{"award-number":["2016YFC1303100"]}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Previous studies have revealed that loss of cell apical\u2010basal polarity contributed to the early stages of tumorigenesis. Adenomyosis involves a down\u2010growth and aberrant implantation of the endometrial basalis into the myometrium. This study discovered aberrant expression of polarity protein Scribble (Scrib) and Crumbs homologue 3 protein (CRB3) in epithelial cells of diffuse adenomyosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a case\u2010controlled study, including 39 patients with histologic evidence of adenomyosis, and 48 patients with carcinoma in situ of the uterine cervix without adenomyosis or endometriosis as control. Adenomyotic foci, eutopic endometrium of adenomyotic patients as well as normal endometrium were collected. Reverse Transcription Polymerase Chain Reaction (RT\u2010PCR), Immunoreactivity, confocal microscopy and immune electron microscopy were conducted to evaluate Scribble expression and localization of Scribble and CRB3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Scrib was screen out as an abnormally expressed polarity protein in adenomyotic eutopic endometrium (ADM\u2010EU) at messenger RNA (mRNA) level. The ADM\u2010EU and adenomyotic ectopic endometrium showed a significantly decreased expression of Scrib compared with normal endometrium (all <jats:italic>P<\/jats:italic>\u2010values &lt;0.05). Scrib decreased significantly in ADM\u2010EU than normal endometrium only in patients at proliferative phase and with severe dysmenorrhea <jats:italic>(P<\/jats:italic>\u2010values &lt;0.01, <jats:italic>P<\/jats:italic>\u2010values &lt;0.001 respectively). In ADM\u2010EU, Scrib expression significantly lowered in patients with severe dysmenorrhea than mild dysmenorrhea (<jats:italic>P<\/jats:italic>\u2010values &lt;0.05). Aberrant redistribution of CRB3 from apical to basal lateral membrane portion was also detected in experiments by confocal microscopy immune electron microscopy (all <jats:italic>P<\/jats:italic>\u2010values &lt;0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Basolateral polarity protein Scrib was found decreased significantly in endometrial cells of adenomyosis at mRNA and protein level, compared with normal endometrium. Menstrual phase and severity of dysmenorrhea has an impact on Scrib expression. Scrib decrease was accompanied by aberrant redistribution of CRB3 from apical to basal lateral membrane portion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13952","type":"journal-article","created":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T06:51:19Z","timestamp":1553583079000},"page":"1148-1159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Decreased expression of cell polarity protein Scribble correlated with altered subcellular localization of the Crumbs homologue 3 protein in human adenomyotic endometrial cells"],"prefix":"10.1111","volume":"45","author":[{"given":"Xiaoyi","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology of Shanghai Medical School, Obstetrics and Gynecology Hospital Fudan University  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwen","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology of Shanghai Medical School, Obstetrics and Gynecology Hospital Fudan University  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiou","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology of Shanghai Medical School, Obstetrics and Gynecology Hospital Fudan University  Shanghai People's Republic of China"},{"name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zehua","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7954-5892","authenticated-orcid":false,"given":"Congjian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology of Shanghai Medical School, Obstetrics and Gynecology Hospital Fudan University  Shanghai People's Republic of China"},{"name":"Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.12.030"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2015.09.018"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.06.027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2006.01.016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.4.312"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.06.044"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2017.06.016"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det285"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7214"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3169"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2008.01.001"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3775"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00500"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02898"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.341"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208632"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/bse0530141"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.129387"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-6814"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2017.24"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.15713"},{"key":"e_1_2_7_23_1","first-page":"842","article-title":"Cytoplasmic localization of the cell polarity factor scribble supports liver tumor formation and tumor cell invasiveness","volume":"6","author":"Wan S","year":"2017","journal-title":"Hepatology"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu046"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/den484"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12339"},{"key":"e_1_2_7_27_1","first-page":"137","article-title":"Endometrial stem\/progenitor cells: The first 10 years","volume":"22","author":"Gargett CE","year":"2016","journal-title":"Hum Reprod Update"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719116650758"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2016.05.012"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.09.045"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.02.051"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/35001108"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.12.014"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201002047"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.174235"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.09.032"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.06.011"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2011.60"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-010-9180-2"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13952","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13952","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jog.13952","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T08:46:52Z","timestamp":1694335612000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.13952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,25]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/jog.13952"],"URL":"https:\/\/doi.org\/10.1111\/jog.13952","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,25]]},"assertion":[{"value":"2018-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}