{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:54:36Z","timestamp":1759964076290,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002386","name":"Cairo University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010794","name":"Faculty of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>As angiogenesis is an essential step for chorionic villi formation. Vascular endothelial growth factor (VEGF) is essential for endothelial cell proliferation. Endothelial nitric oxide synthase (eNOS) is a powerful playmaker in hypoxia\u2010induced angiogenesis. Thrombin\u2010activatable fibrinolysis inhibitor (TAFI) regulates both fibrinolysis and inflammation. Genetic alterations of these factors may lead to recurrent spontaneous abortion (RSA). We aimed to investigate the combined genetic variants of VEGF G\u20101154A and two eNOS genetic variants: T\u2010786C promoter region and intron 4 variable number of tandom repeats in addition to TAFI C\u20101040T among RSA patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 50 patients with RSA and 50 healthy controls. Polymerase chain reaction and restriction fragment length polymorphism were used for genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both genetic alterations of eNOS confirmed at least a sixfold increase of RSA risk. Interestingly, they were associated with TAFI C\u20101040Tgenetic variant in 21 patients, eight of them had both studied eNOS genetic alterations and TAFI C\u20101040Tgenetic variant, while each eNOS genetic variant associated with TAFI C\u20101040Tconfirmed an almost one and half fold increase risk of RSA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings highlighted the role of eNOS and nitric oxide metabolism in RSA and opened the gate to investigate the interaction of vasoconstrictive and fibrinolytic inhibitor systems.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13961","type":"journal-article","created":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T07:52:40Z","timestamp":1554882760000},"page":"1106-1113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The prevalence of combined vascular endothelial growth factor, endothelial nitric oxide synthase and thrombin\u2010activatable fibrinolysis inhibitor genetic polymorphisms among Egyptian patients with recurrent spontaneous abortion"],"prefix":"10.1111","volume":"45","author":[{"given":"Nelly N.","family":"Abulata","sequence":"first","affiliation":[{"name":"Department of Clinical and Chemical Pathology Kasr Al\u2010Ainy Faculty of Medicine, Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5525-9250","authenticated-orcid":false,"given":"Iman A.","family":"Shaheen","sequence":"additional","affiliation":[{"name":"Department of Clinical and Chemical Pathology Kasr Al\u2010Ainy Faculty of Medicine, Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omneya M.","family":"Osman","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kasr Al\u2010Ainy Faculty of Medicine, Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kasr Al\u2010Ainy Faculty of Medicine, Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waleed M.","family":"El\u2010Khayat","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kasr Al\u2010Ainy Faculty of Medicine, Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,10]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"441","article-title":"Recurrent spontaneous early pregnancy loss and low dose aspirin","volume":"55","author":"Daya S","year":"2003","journal-title":"Minerva Gynecol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.01.026"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.5.1345"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/8.5.463"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"e0123696","DOI":"10.1371\/journal.pone.0123696","article-title":"Association of VEGF genetic polymorphisms with recurrent spontaneous abortion risk: A systematic review and meta\u2010analysis","volume":"4","author":"Xu X","year":"2015","journal-title":"PLoS One"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1022795416080081"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029617695485"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1452"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2012.01123.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1038\/nm0603-669","article-title":"The biology of VEGF and it's receptors","volume":"9","author":"Ferrara N","year":"2002","journal-title":"Nat Med"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.4.617"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/6.3.276"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2015.2292"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)02191-K"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2015.09.002"},{"key":"e_1_2_7_17_1","first-page":"33","article-title":"Thrombin activatable fibrinolysis inhibitor (TAFI) in cord blood","volume":"45","author":"Uszynski W","year":"2007","journal-title":"Folia Histochem Cytobiol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr15049060"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2298\/GENSR1602707P"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2007.00515.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmr027"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-008-0577-8"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080940"},{"key":"e_1_2_7_24_1","first-page":"67","article-title":"Vascular endothelial growth factor gene polymorphism in Egyptian women with recurrent spontaneous miscarrage","volume":"1","author":"Abulata N","year":"2013","journal-title":"Egypt J Lab Med"},{"key":"e_1_2_7_25_1","first-page":"629","article-title":"Endothelial nitric oxide synthase gene polymorphism in a cohort of Egyptian women with recurrent spontaneous miscarriage","volume":"3","author":"Abulata NN","year":"2014","journal-title":"Comp Clin Pathol"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2011.01067.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1191\/0961203304lu516oa"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/16.8.1644"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800254-4.00015-5"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-005-0107-x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/14647273.2013.806824"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2010.05.014"},{"key":"e_1_2_7_33_1","first-page":"299","article-title":"Assessment of genetic factors for type 2 diabetes mellitus","volume":"18","author":"Yamada Y","year":"2006","journal-title":"Int J Mol Med"},{"key":"e_1_2_7_34_1","first-page":"220","article-title":"Assessment of genetic risk for myocardial infarction","volume":"2","author":"Yamada Y","year":"2006","journal-title":"Thromb Haemost"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.10.020"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.041359198"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12881-015-0234-6"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01375.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977194.2015.1054990"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601579"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.13961","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13961","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jog.13961","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13961","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T08:46:40Z","timestamp":1694335600000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.13961"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,10]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/jog.13961"],"URL":"https:\/\/doi.org\/10.1111\/jog.13961","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"type":"print","value":"1341-8076"},{"type":"electronic","value":"1447-0756"}],"subject":[],"published":{"date-parts":[[2019,4,10]]},"assertion":[{"value":"2018-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}