{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:27:34Z","timestamp":1781008054931,"version":"3.54.1"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T00:00:00Z","timestamp":1559865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study is to determine the association between C677T polymorphism in <jats:italic>MTHFR<\/jats:italic> gene and plasma homocysteine concentration in recurrent fetal miscarriages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Overall, 100 women were included in the research, the case group comprised of 50 women who had a history of spontaneously recurrent miscarriage with unspecified cause, and 50 of whom had experienced at least two successful pregnancy, as controls. Methods used in the study included PCR\u2010RFLP with limited effective HinfI enzyme in order to investigate <jats:italic>MTHFR<\/jats:italic> polymorphism and enzyme\u2010linked immunosorbent assay analysis to investigate plasma homocysteine concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was a significant increase in the prevalence of mutant TT genotype among women with miscarriage history. Also, the mean homocysteine level in the case group was significantly higher than that in the control group (<jats:italic>P<\/jats:italic>\u2009=\u20090.002) and higher level of homocysteine was found in the carriers of T allele.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest that C677TT genotype may be a risk factor for miscarriage and CC wild type genotype supposed to have protective effect on hyperhomocysteinemia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.13989","type":"journal-article","created":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T08:54:00Z","timestamp":1559897640000},"page":"1442-1447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Investigation of the association between C677T polymorphism of the <i>MTHFR<\/i> gene and plasma homocysteine level in recurrent fetal miscarriage"],"prefix":"10.1111","volume":"45","author":[{"given":"Yalda","family":"Zarfeshan Fard","sequence":"first","affiliation":[{"name":"Department of Genetics, Faculty of Basic Sciences Sistan and Baluchistan University  Zahedan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omid","family":"Kooshkaki","sequence":"additional","affiliation":[{"name":"Department of Immunology Birjand University of Medical Sciences  Birjand Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dormohammad","family":"Kordi Tammandani","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Basic Sciences Sistan and Baluchistan University  Zahedan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamreza","family":"Anani Sarab","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center Birjand University of Medical Sciences  Birjand Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/09723757.2006.11885950"},{"key":"e_1_2_7_3_1","first-page":"16","volume-title":"Unsafe Abortion: Global and Regional Estimates of the Incidence of Unsafe Abortion and Associated Mortality in 2008","author":"World Health Organization.","year":"2011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg030"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.082764gb"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmg013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(00)00404-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.8755-8920.2001.450202.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.14310\/horm.2002.1505"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1016220"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.109.184127"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seizure.2005.11.002"},{"key":"e_1_2_7_13_1","first-page":"12","article-title":"Pathobiology and genetics of neural tube defects","volume":"4","author":"Finnell R","year":"2003","journal-title":"Epilepsia"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2007.01.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-1211(01)00288-1"},{"key":"e_1_2_7_16_1","first-page":"20","article-title":"Molecular biology of 5, 10\u2010methylenetetrahydrofolate reductase","volume":"13","author":"F\u00f6dinger M","year":"2000","journal-title":"J Nephrol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(00)00426-7"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1515\/jpm-2012-0252","article-title":"MTHFR C677T polymorphism, folate, vitamin B12 and homocysteine in recurrent pregnancy losses: A case control study among north Indian women","volume":"41","author":"Puri M","year":"2013","journal-title":"J Perinat Med"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-9560-5-10"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(01)01789-6"},{"key":"e_1_2_7_21_1","first-page":"238","article-title":"C677T and A1298C mutation of the methylenetetrahydrofolate reductase gene in unexplained recurrent spontaneous abortion","volume":"39","author":"Wang XP","year":"2004","journal-title":"Zhonghua Fu Chan Ke Za Zhi"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00815"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"587","DOI":"10.18388\/abp.2008_3065","article-title":"Methylenetetrahydrofolate reductase (MTHFR\u2010677 and MTHFR\u20101298) genotypes and haplotypes and plasma homocysteine levels in patients with occlusive artery disease and deep venous thrombosis","volume":"55","author":"Spiroski I","year":"2008","journal-title":"Acta Biochim Pol"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.18203\/2320-1770.ijrcog20172013"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i4.1081"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13989","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jog.13989","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.13989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T03:55:14Z","timestamp":1694318114000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.13989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,7]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1111\/jog.13989"],"URL":"https:\/\/doi.org\/10.1111\/jog.13989","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,7]]},"assertion":[{"value":"2018-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}