{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:24:01Z","timestamp":1774679041772,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Infants with fetal growth restriction (FGR) are at an increased risk of perinatal morbidity and mortality. The long noncoding RNA <jats:italic>H19<\/jats:italic> gene is expressed abundantly in placental villi and recent studies suggest that it regulates FGR. However, the role of <jats:italic>H19<\/jats:italic> in the FGR placenta remains unclear. This study aimed to clarify the relationship between <jats:italic>H19<\/jats:italic> expression and FGR using normotensive placentas after 34\u2009weeks of gestation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Formalin\u2010fixed paraffin\u2010embedded tissues from human placentas collected from pregnancies resulting in small for gestational age (SGA) and appropriate for gestational age (AGA) newborns were used. The histopathological features of placenta tissues, such as villous stromal fibrosis, the numbers of terminal villi, villous vessels and cytotrophoblasts were analyzed using hematoxylin and eosin, Masson's trichrome staining and immunostaining. The localization and expression of <jats:italic>H19<\/jats:italic> in the placentas were demonstrated by <jats:italic>in situ<\/jats:italic> hybridization and reverse transcription\u2010quantitative polymerase chain reaction (RT\u2010qPCR), respectively. Moreover, the expression levels of <jats:italic>H19<\/jats:italic>\u2010regulated molecules such as <jats:italic>IGF2<\/jats:italic> and decorin (<jats:italic>DCN<\/jats:italic>) were measured by RT\u2010qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histopathological features of the placental villous were not different between placentas associated with SGA and AGA. <jats:italic>H19<\/jats:italic> localized to the villous stroma, endothelial cells and cytotrophoblasts. Moreover, the expression level of <jats:italic>H19<\/jats:italic> in SGA placentas was significantly lower than that in AGA placentas. The expression levels of <jats:italic>IGF2<\/jats:italic> and <jats:italic>DCN<\/jats:italic> in SGA placentas tended to be lower than those in AGA placentas similarly to <jats:italic>H19<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study highlights the potential importance of regulatory events mediated by <jats:italic>H19<\/jats:italic> in SGA placentas without histopathological abnormalities in late pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.14260","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T05:40:47Z","timestamp":1587620447000},"page":"1025-1034","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Expression level of long noncoding <scp>RNA <i>H19<\/i><\/scp> of normotensive placentas in late pregnancy relates to the fetal growth 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