{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T08:54:53Z","timestamp":1778662493968,"version":"3.51.4"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Postmenopausal osteoporosis is a systemic and chronic bone disease in women. In order to understand the pathological mechanism of postmenopausal osteoporosis, we aimed to find the potential differentially expressed miRNAs in the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Firstly, RNA sequencing was used to identify differentially expressed miRNAs, followed by the construction of the miRNA\u2013target mRNA regulatory network. Then, Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes were used to analyze the biological function of target mRNAs. Finally, electronic validation of identified differentially expressed miRNAs and target mRNAs was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 33 differentially expressed miRNAs (18 upregulated and 15 downregulated miRNAs) and 6820 miRNA\u2013mRNA pairs were identified. Among which, seven miRNAs with high degree including hsa\u2010miR\u201017\u20105p, hsa\u2010miR\u20101\u20103p, hsa\u2010miR\u2010193b\u20103p, hsa\u2010miR\u2010125b\u20105p, hsa\u2010miR\u201010b\u20105p, hsa\u2010miR\u2010100\u20105p and hsa\u2010miR\u201030a\u20103p were obtained in the miRNA\u2013mRNA regulatory network. TGF\u2010beta was the most significantly enriched signaling pathway of target mRNAs. The electronic validation result of hsa\u2010miR\u20101\u20103p, hsa\u2010miR\u2010193b\u20103p, hsa\u2010miR\u201010b\u20105p, hsa\u2010miR\u2010100\u20105p, hsa\u2010miR\u2010133b, hsa\u2010miR\u2010708\u20105p, CRK, RAB5C, CCND1 and PCYOX1 was consisted with the RNA sequencing analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Dysfunctional miRNAs may play significant roles in postmenopausal osteoporosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.14307","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T23:42:33Z","timestamp":1589931753000},"page":"1183-1192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Searching for valuable differentially expressed <scp>miRNAs<\/scp> in postmenopausal osteoporosis by <scp>RNA<\/scp> 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