{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:54:04Z","timestamp":1776016444456,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008630","name":"Children's Health Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008630","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MFM\u2010146443"],"award-info":[{"award-number":["MFM\u2010146443"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Obesity has been associated with changes in autophagy and its increasing prevalence among pregnant women is implicated in higher rates of placental\u2010mediated complications of pregnancy such as pre\u2010eclampsia and intrauterine growth restriction. Autophagy is involved in normal placentation, thus changes in autophagy may lead to impaired placental function and development. The aim of this study was to investigate the connection between obesity and autophagy in the placenta in otherwise uncomplicated pregnancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Immunohistochemistry and western blot analysis were done on placental and omental samples from obese (body mass index [BMI] \u226530\u2009kg\/m<jats:sup>2<\/jats:sup>) and normal weight (BMI &lt;25\u2009kg\/m<jats:sup>2<\/jats:sup>) pregnant women with singleton pregnancies undergoing planned Caesarean delivery without labor at term. Samples were analyzed for autophagic markers LC3B and p62 in the peripheral, middle and central regions of the placenta and in omental adipocytes, milky spots and vasculature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As pre\u2010pregnancy BMI increased, there was an increase in both placental and fetal weight as well as decreased levels of LC3B in the central region of the placenta (<jats:italic>P<\/jats:italic> = 0.0046). Within the obese patient group, LC3B levels were significantly decreased in the placentas of male fetuses compared to females (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). Adipocytes, compared to milky spots and vasculature, had lower levels of p62 (<jats:italic>P<\/jats:italic> = 0.0127) and LC3B (<jats:italic>P<\/jats:italic> = 0.003) in obese omenta and lower levels of LC3B in control omenta (<jats:italic>P<\/jats:italic> = 0.0071).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Obesity leads to reduced placental autophagy in uncomplicated pregnancies; thus, changes in autophagy may be involved in the underlying mechanisms of obesity\u2010related placental diseases of pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.14315","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T23:59:55Z","timestamp":1590710395000},"page":"1282-1291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Maternal obesity reduces placental autophagy marker expression in uncomplicated pregnancies"],"prefix":"10.1111","volume":"46","author":[{"given":"Matthew","family":"Cohen","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Schulich School of Medicine and Dentistry The University of Western Ontario  London Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Schulich School of Medicine and Dentistry The University of Western Ontario  London Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aidan","family":"Pucchio","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Schulich School of Medicine and Dentistry The University of Western Ontario  London Ontario Canada"},{"name":"The Mary &amp; John Knight Translational Ovarian Cancer Research Unit, Lawson Health Research Institute  London Ontario 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