{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T12:35:51Z","timestamp":1718886951801},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,23]],"date-time":"2020-08-23T00:00:00Z","timestamp":1598140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur\u2010containing foods such as garlic on rat uterine activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague\u2013Dawley rats (250\u2013300\u2009g) in estrus stage of estrous cycle were studied in isolated organ baths containing Krebs\u2013Henseleit solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Diallyl sulfide (10<jats:sup>\u22128<\/jats:sup>\u201310<jats:sup>\u22126<\/jats:sup>\u2009M) caused a concentration\u2010dependent relaxation on KCl (60\u2009mM)\u2010induced contractions and inhibited spontaneous peristaltic activity of uterine strips (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). None of the following antagonists significantly changed the inhibitory effect of DAS on both KCl\u2010precontracted uterine strips and spontaneous peristaltic activity of the uterus (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05): nitric oxide synthase inhibitor L\u2010NAME (10<jats:sup>\u22124<\/jats:sup>\u2009M), hydrogen sulfide\u2010producing enzymes cystation \u03b2 synthase and cystation \u03b3\u2010lyase inhibitors, aminooxyacetic acid (10<jats:sup>\u22124<\/jats:sup>\u2009M) and propargylglycine (10<jats:sup>\u22123<\/jats:sup>\u2009M) and nonselective cyclooxygenase inhibitor indomethacin (10<jats:sup>\u22124<\/jats:sup>\u2009M). However, in calcium\u2010free Krebs solution containing high KCl (30\u2009mM), DAS significantly inhibited CaCl<jats:sub>2<\/jats:sub> (10<jats:sup>\u22125<\/jats:sup>\u201310<jats:sup>\u22122<\/jats:sup>\u2009M)\u2010induced uterine contractions in a concentration\u2010dependent manner (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Diallyl sulfide has a relaxing effect on KCl\u2010contracted rat uterus strips and an inhibitory effect on spontaneous uterine activity, possibly by decreasing the calcium influx into the cytoplasm of uterine smooth muscle cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.14421","type":"journal-article","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T02:32:17Z","timestamp":1598236337000},"page":"88-95","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage\u2010dependent calcium channels"],"prefix":"10.1111","volume":"47","author":[{"given":"O\u011fuzhan E.","family":"Efe","sequence":"first","affiliation":[{"name":"Department of Pharmacology Baskent University Faculty of Medicine  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Michael","family":"Lux","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Baskent University Faculty of Medicine  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selda","family":"Emre Ayding\u00f6z","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Baskent University Faculty of Medicine  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meral","family":"Tuncer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Baskent University Faculty of Medicine  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.11682.1"},{"key":"e_1_2_7_3_1","first-page":"81","article-title":"Dysmenorrhea and endometriosis in young women","volume":"56","author":"Harada T","year":"2013","journal-title":"Yonago Acta Med"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmq016"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.5152\/jtgga.2013.67763"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/eph8602114"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.suppl_1.81"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cophys.2019.11.010"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2009.03.005"},{"key":"e_1_2_7_10_1","first-page":"70","article-title":"The physiological mechanism of uterine contraction with emphasis on calcium ion","volume":"1","author":"Otaibi MA","year":"2014","journal-title":"Calcium Signal"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/eph8602179"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20474"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods8070246"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2016.11.016"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2018.07.009"},{"key":"e_1_2_7_16_1","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"National Research Council","year":"2011"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623315570339"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40738-020-00074-3"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2007.038125"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27069-x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12387.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2007.05.002"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2016.07.016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.2147\/IBPC.S51434"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12171"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.14421","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jog.14421","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.14421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T05:16:50Z","timestamp":1693718210000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.14421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,23]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1111\/jog.14421"],"URL":"https:\/\/doi.org\/10.1111\/jog.14421","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,23]]},"assertion":[{"value":"2020-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}