{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:37:39Z","timestamp":1781822259302,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Exosomes present essential roles for intercellular interaction via extracellular pathways during systemic dysfunctions, including preeclampsia (PE). Here, we assessed the specific mechanism of mesenchymal stem cells (MSC)\u2010originated exosomes in PE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The effects of exosomes on trophoblasts were studied by EdU, wound healing, Transwell and TUNEL assays. By microarray analysis, we found that exosomes enhanced the microRNA\u2010139\u20105p (miR\u2010139\u20105p) in trophoblasts, and confirmed the target gene of miR\u2010139\u20105p by bioinformatics prediction and dual\u2010luciferase reporter gene assay. At the same time, ERK\/MMP\u20102 pathway\u2010related biomolecules were assessed through Western blot analysis. The pathway inhibitor was used for rescue experiments. Finally, the effect of exosomes on the pathology of PE rats was verified by <jats:italic>in vivo<\/jats:italic> experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The exosomes originated from hucMSC fostered the trophoblast cell migration, invasion and proliferation and obstructed apoptosis. Moreover, miR\u2010139\u20105p could be transmitted to trophoblasts through hucMSC\u2010secreted exosomes. miR\u2010139\u20105p targeted protein tyrosine phosphatase (PTEN), which regulated the ERK\/MMP\u20102 pathway. Inhibition of the ERK\/MMP\u20102 pathway significantly reduced the promoting effect of exosomes on trophoblasts. Treatment with exosomes significantly lowered blood pressure values and reduced 24\u2010h proteinuria in PE rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>hucMSC\u2010originated exosomes overexpressing miR\u2010139\u20105p activated the ERK\/MMP\u20102 pathway via PTEN downregulation, thus accelerating trophoblast cell invasion and migration, and blocking apoptosis. These results demonstrated that hucMSC\u2010derived exosomes overexpressing miR\u2010139\u20105p might be an innovative direction for therapeutic approaches against PE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.14495","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T02:30:56Z","timestamp":1600396256000},"page":"2561-2572","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Exosomal <scp>microRNA<\/scp>\u2010139\u20105p from mesenchymal stem cells accelerates trophoblast cell invasion and migration by motivation of the <scp>ERK<\/scp>\/<scp>MMP<\/scp>\u20102 pathway via downregulation of <scp>protein tyrosine phosphatase<\/scp>"],"prefix":"10.1111","volume":"46","author":[{"given":"Huijie","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Obstetrics Liaocheng Second People's Hospital  Liaocheng Shandong 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