{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:23:24Z","timestamp":1778570604168,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Polycystic ovary syndrome (PCOS) is a complicated endocrine and metabolic abnormality diseases common in women of child\u2010bearing age. This study aims to screen out critical miRNAs and mRNAs associated with PCOS, which may be conducive to offer novel insights and treatment for the diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three mRNA datasets and one miRNA dataset derived from granulosa cells of patients with PCOS and normal controls were downloaded to obtain the differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs). Then, DEmiRNA\u2010target DEmRNAs analysis and functional annotation of DEmiRNA\u2010target DEmRNAs were performed. Quantitative real time polymerase chain reaction (qRT\u2010PCR) validation of the expression of the selected DEmRNAs and DEmiRNAs were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 1643 DEmRNAs, 88 DEmiRNAs, 2406 DEmiRNA (down)\u2010DEmRNA (up), and 2179 DEmiRNA (up)\u2010DEmRNA (down) pairs were obtained. The functional annotation of DEmiRNA\u2010target DEmRNAs revealed that C\u2010type lectin receptor signaling pathway, Steroid biosynthesis and Galactose metabolism were significantly enriched KEGG pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings may provide make contribution to understanding PCOS pathogenesis, diagnosis, or treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.14707","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T18:14:50Z","timestamp":1613672090000},"page":"1416-1424","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of critical <scp>miRNAs<\/scp> and <scp>mRNAs<\/scp> associated with polycystic ovary syndrome"],"prefix":"10.1111","volume":"47","author":[{"given":"Xinghua","family":"Diao","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongliang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaixue","family":"Lao","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9119-0476","authenticated-orcid":false,"given":"He","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Binzhou Medical University Hospital  Binzhou Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2018.04.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2016.05.009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/js.2019-00078"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv029"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.5812\/ijem.91554"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/TACG.S200341"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2019.04.082"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.003"},{"issue":"6","key":"e_1_2_7_10_1","first-page":"902","article-title":"MicroRNA\u2010145 negatively regulates cell proliferation through targeting IRS1 in isolated ovarian granulosa cells from patients with polycystic ovary syndrome. 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