{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:59:01Z","timestamp":1764784741301,"version":"3.40.5"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T00:00:00Z","timestamp":1632700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Both human and rat myometrium express stromal interaction molecule (STIM) and Orai\/transient receptor potential canonical (TRPC) proteins, which are components of plasma membrane Ca<jats:sup>2+<\/jats:sup> store\u2010operated channels. There are reports that these proteins mediate agonist\u2010induced Ca<jats:sup>2+<\/jats:sup> influx in cultured myometrial cells. In this study, we aimed to determine the effects of Pyr6, an Orai channel blocker, on different agonist\u2010induced contractions in isolated segments of rat uterus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main findings<\/jats:title><jats:p>In Ca<jats:sup>2+<\/jats:sup>\u2010free Tyrode's solution, Pyr6 (3\u00a0\u03bcM) promoted a reduction in both the magnitude and frequency of Ca<jats:sup>2+<\/jats:sup> (1\u2009mM)\u2010induced uterine contractions after the addition of carbachol (CCh, 100\u2009\u03bcM), but not after the addition of oxytocin (OT, 150\u2009nM). In Ca<jats:sup>2+<\/jats:sup> (0.18\u2009mM)\u2010Tyrode's solution, Pyr6 completely relaxed uterine contractions induced by both CCh and cloprostenol (300\u2009nM), but not those induced by either KCI (40\u201380\u2009mM) or OT. The addition of Pyr6 abolished the oscillatory uterine contractions induced by Ca<jats:sup>2+<\/jats:sup> after the addition of cyclopiazonic acid (CPA, 10\u00a0\u03bcM). When pre\u2010incubated (5\u00a0min), Pyr6 reduced the magnitude of both CCh\u2010induced phasic and tonic contractions. The addition of Pyr2 (3\u00a0\u03bcM), an Orai and TRPC channel blocker, abolished uterine contractions induced by CCh or OT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Considering Pyr6 as an Orai channel blocker and its inhibitory effect on uterine contractions induced by CCh, CPA, and cloprostenol, we suggest that Orai channels are required for the maintenance of contractions induced by these agonists in rat uterus.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15034","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T02:05:48Z","timestamp":1632794748000},"page":"4306-4318","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Inhibitory effect of Pyr6 (an Orai channel blocker) on <scp>agonist\u2010induced<\/scp> contractions in rat uterus"],"prefix":"10.1111","volume":"47","author":[{"given":"\u00cdcaro Ara\u00fajo","family":"de Sousa","sequence":"first","affiliation":[{"name":"Biophysics and Physiology Department Health Sciences Center, Federal University of Piau\u00ed, Ininga  Teresina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Medina Sobreira","family":"de Meneses","sequence":"additional","affiliation":[{"name":"Biophysics and Physiology Department Health Sciences Center, Federal University of Piau\u00ed, Ininga  Teresina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Victor Miranda","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Biophysics and Physiology Department Health Sciences Center, Federal University of Piau\u00ed, Ininga  Teresina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo Queiroz","family":"Lopes","sequence":"additional","affiliation":[{"name":"Pharmacological Sciences Department Health Sciences Center Federal University of Para\u00edba, Cidade Universit\u00e1ria \u2010 Campus I. 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