{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:25:01Z","timestamp":1790022301053,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,17]],"date-time":"2021-10-17T00:00:00Z","timestamp":1634428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Microbiota of the reproductive tract may be associated with unexplained infertility in women. We aimed to determine the derangements of vaginal and endometrial microbiota related to unexplained infertility by real\u2010time polymerase chain reaction (real\u2010time PCR) microbiota analyses of vaginal and endometrial samples of the unexplained infertility patients and fertile women.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Twenty\u2010six women with unexplained infertility and 26 age\u2010matched fertile women were included. Vaginal and endometrial samples were obtained in the mid\u2010menstrual cycle for analysis by quantitative real\u2010time PCR method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The proportion of lactobacilli\u2010impaired microbiota was significantly higher in the vaginal samples of unexplained infertility patients (76.9% vs. 26.9%; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Those with impaired lactobacilli microbiota of vaginal samples had an increased risk of 9.048 times for infertility than those with normal lactobacilli microbiota. In addition, the mean lactobacilli\/total bacterial mass (TBM) ratio in the vaginal samples was significantly lower in the unexplained infertility patients (38.2% vs.76.3%; <jats:italic>p<\/jats:italic>\u00a0=\u00a00.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present study results supported the role of vaginal and endometrial microbiota derangements in unexplained infertility. Many time\u2010consuming and invasive methods are currently used in the diagnosis of infertility. Our study showed that the quantitative determination of lactobacilli\/TBM ratio in vaginal specimens, a less invasive and easily obtainable method, could be used as a diagnostic test during the workup of couples with unexplained infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15060","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T21:46:48Z","timestamp":1634766408000},"page":"129-139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Assessment of vaginal and endometrial microbiota by real\u2010time <scp>PCR<\/scp> in women with unexplained infertility"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5727-7965","authenticated-orcid":false,"given":"Ozlem","family":"Sezer","sequence":"first","affiliation":[{"name":"Department of Medical Genetics Samsun Education and Research Hospital, University of Health Sciences  Samsun 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