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Histological alternations can be observed in placenta, but placental transcriptome profile and circular RNAs have not been studied in this disease. The aim of this study was to define the placental transcriptional changes and find relevant circular RNAs in BHFS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed high\u2010throughput RNA sequencing to detect placental samples from fetuses affected by BHFS (<jats:italic>n<\/jats:italic>\u00a0=\u00a05) and normal fetuses (NF, <jats:italic>n<\/jats:italic>\u00a0=\u00a05), quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR), and Sanger sequencing to validate the differentially expressed circRNAs and their potentially related miRNAs (BHFS, <jats:italic>n<\/jats:italic>\u00a0=\u00a022; NF, <jats:italic>n<\/jats:italic>\u00a0=\u00a011). Bioinformatics methods were performed for further analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results showed 152 differentially expressed genes (DEGs), 112 circRNAs, and 45 microRNAs that were differentially expressed. DEGs were found to be involved in Gene Ontology terms related to gas transport, cell adhesion, oxidative stress, organ development, hemopoiesis, and others. RT\u2010qPCR results showed that hsa_circ_0003961 and hsa_circ_0006687 were upregulated (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). The competing endogenous RNA and co\u2010expression networks showed that hsa_circ_0003961 and hsa_circ_0006687 were connected with 3 miRNAs and some DEGs, including cell adhesion genes (e.g., <jats:italic>CLDN19<\/jats:italic>), hemoglobin related genes (e.g., <jats:italic>SOX6<\/jats:italic> and <jats:italic>HBZ<\/jats:italic>) and angiogenesis related genes (e.g., <jats:italic>EPHB2<\/jats:italic>). Downregulations of hsa\u2010miR\u20101299 and hsa\u2010miR\u2010625\u20105p in ceRNA network were also validated by RT\u2010qPCR. Gene set enrichment analysis results for the two circRNAs showed that some gene sets associated with cell adhesion, hematopoietic system and apoptosis were significantly enriched.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study characterized the placental transcriptome of BHFS. The circRNAs hsa_circ_0003961 and hsa_circ_0006687 in placenta may be relevant to BHFS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15126","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T23:19:42Z","timestamp":1640128782000},"page":"313-327","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Placental transcriptome sequencing combined with bioinformatics predicts potential genes and circular <scp>RNAs<\/scp> associated with hemoglobin Bart's hydrops fetalis syndrome"],"prefix":"10.1111","volume":"48","author":[{"given":"Lingjie","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Prenatal Diagnosis and Genetic Diseases The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yebin","family":"Lu","sequence":"additional","affiliation":[{"name":"Guangxi Medical University  Nanning 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