{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T21:23:34Z","timestamp":1771104214127,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To introduce a quantitative determination of heparan sulfate and dermatan sulfate by mass spectrometry and to compare it with two\u2010dimensional electrophoresis of the glycosaminoglycans in the amniotic fluid for the prenatal diagnosis of mucopolysaccharidoses type II (MPS II).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty pregnancies each with single fetus were subjected to amniocentesis at 16\u2009weeks: 10 with a previously affected MPS II infant and 20 as controls. Prenatal diagnosis was done by both mass spectrometry two two\u2010dimensional electrophoresis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two\u2010dimensional electrophoresis showed four affected with MPS II and six unaffected fetuses. Mass spectrometry verified these results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Two\u2010dimensional electrophoresis of the glycosaminoglycans in amniotic fluid is a good qualitative method and mass spectrometry is a new accurate quantitative method for prenatal diagnosis of MPS II. Quantitative determination of glycosaminoglycans in amniotic fluid by mass spectrometry is both rapid and accurate. Prenatal diagnosis is recommended for at risk pregnancies and mass spectrometry offers speed and quantitation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15135","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T23:19:01Z","timestamp":1642115941000},"page":"682-687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prenatal diagnosis of mucopolysaccharidoses type <scp>II<\/scp> by two\u2010dimensional electrophoresis and mass spectrometry in amniotic fluid"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6770-5089","authenticated-orcid":false,"given":"Aly A.","family":"Aboulnasr","sequence":"first","affiliation":[{"name":"Prenatal Diagnosis and Fetal Medicine Department National Research Centre  Giza 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