{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T13:31:21Z","timestamp":1785159081811,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify trophoblastic cells retrieved from the cervix at a gestational age (GA) of 5\u20139\u2009weeks by a noninvasive modality in fetuses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Transcervical cells (TCCs) were noninvasively extracted by a cytobrush using the Papanicolaou sampling method. TCCs were immunostained with antihuman leukocyte antigen (HLA)\u2010G and anticytokeratin (CK)\u20107 antibodies to identify trophoblastic cells. Maternal finger blood, gestational sacs, and 20 trophoblastic cells collected by a laser\u2010guided microscopic single\u2010cell capture system were examined and compared by short tandem repeat (STR) genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010nine pregnant women with GA of 5\u20139\u2009weeks and six nonpregnant healthy women were included in the study. Trophoblastic cells were identified in 37 (75.5%) TCC samples, among which 34 (69.4%) were eligible for STR genotyping analysis. No trophoblastic cells were identified in nonpregnant healthy women. The STR genotyping analyses revealed 24 female and 10 male fetuses. TCC trophoblastic cells exhibited the same STR profiles as gestational sac and maternal blood in all samples, which indicated that the TCC trophoblastic cells originated from fetuses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This primary study validated that trophoblastic cells from TCCs at GA 5\u20139\u2009weeks originated from the fetus. Further studies are needed to verify whether this method can be used for early noninvasive prenatal diagnosis and paternity testing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15256","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T07:35:25Z","timestamp":1650612925000},"page":"1613-1620","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Noninvasive isolation of transcervical trophoblast cells for fetal identification"],"prefix":"10.1111","volume":"48","author":[{"given":"Yi","family":"Gao","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Department the 8<sup>th<\/sup> People's Hospital of Shanghai  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Ruan","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department the 8<sup>th<\/sup> People's Hospital of Shanghai  Shanghai 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