{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T19:00:59Z","timestamp":1793300459891,"version":"4.5.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a bleeding disease that can cause fetal hydrops, a rare but life\u2010threatening condition in which abnormal amounts of fluid accumulate in one or two areas of the fetus's body. A case of FNAIT with fetal hydrops caused by anti\u2010HPA\u201015b antibodies was involved in this study, as we investigated whether or not anti\u2010HPA\u201015b antibodies can induce endothelial angiogenesis and apoptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The monoclonal antibody immobilization of platelet antigens assay (MAIPA) was used to identify anti\u2010HPA\u201015b antibodies. The three groups in Tube formation and apoptosis assays were the PBS group, the AB serum IgG group, and the anti\u2010HPA\u201015b serum IgG group, all reacted with HPA\u201015bb HUVEC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The presence of anti\u2010HPA\u201015b antibodies was found in this case by MAIPA assay. The OD values are 0.33 and 0.21, reacted with HPA\u201015bb and HPA\u201015ab platelets, respectively (cutoff OD value\u2009=\u20090.2). Quantitative analysis revealed that the length of capillary\u2010like tube induced by anti\u2010HPA\u201015b antibodies was significantly decreased over that of AB serum IgG (*\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0005), but weaker than when incubated with thrombin (**\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0009). The apoptosis results show a significantly increased number of apoptotic endothelial cells in the anti\u2010HPA\u201015b antibody IgG group when compared with the PBS and AB serum IgG groups (*\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001, **\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). In addition, there is no statistical difference between the PBS and AB serum groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Anti\u2010HPA\u201015b antibodies can inhibit angiogenesis and induce apoptosis. This may associate with hydrops fetalis (HF), or fetal hydrops of FNAIT.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.15257","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T01:10:37Z","timestamp":1649293837000},"page":"1668-1674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Preliminary mechanism in fetal alloimmune thrombocytopenia associated with\n                    <scp>anti\u2010HPA<\/scp>\n                    15b antibodies"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8214-1082","authenticated-orcid":false,"given":"Yuan","family":"Shao","sequence":"first","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuzhang","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingqin","family":"Mai","sequence":"additional","affiliation":[{"name":"Medical Genetics Centre Guangdong Women and Children Hospital  Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangping","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Medical Genetics Centre Guangdong Women and Children Hospital  Guangdong p.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4681-1947","authenticated-orcid":false,"given":"Wenjie","family":"Xia","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion Guangzhou Blood Centre  Guangzhou Guangdong p.R. 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