{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:23:58Z","timestamp":1762341838033,"version":"3.40.5"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T00:00:00Z","timestamp":1653782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circular RNA plays a critical role in cell proliferation, differentiation, and autophagy. However, the role and mechanism of circRNA in preeclampsia is yet to be clarified. The present study investigated the function and mechanism of circ\u20100002814 in preeclampsia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression level of circ\u20100002814, microRNA 210 (miR210), Notch receptor 1 (Notch1), or cytoplasmic polyadenylation element\u2010binding protein 2 (CPEB2) was investigated by Reverse transcription Real\u2010time Quantitative PCR (RT\u2010qPCR). The level of Notch1, CPEB2, and FUS (FUS RNA\u2010binding protein) proteins was detected by western blot. The localization of circ\u20100002814 and miR\u2010210 was determined by FISH assay. Cell proliferation and invasion were detected by EdU and transwell assays, respectively. The interaction between circ\u20100002814 and FUS protein was detected by RNA pulldown assay, while that between circ\u20100002814 and miR\u2010210 was detected by dual\u2010luciferase reporter assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of circ\u20100002814 was decreased in the placenta and plasma of patients with preeclampsia. The ectopic expression of circ\u20100002814 promoted the invasion and proliferation of HTR8 cells compared to the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Silencing circ\u20100002814 suppressed the invasion and proliferation of JEG3 cells compared to the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). circ_0002814\u2010regulated Notch1 and CPEB2 by interaction with miR\u2010210 and also regulated FUS\/VEGF axis in HTR8 and JEG3 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A low expression of circ\u20100002814 was detected in the plasma and placental tissue in preeclampsia patients. circ\u20100002814 participated in the proliferation and invasion of HTR8 and JEG3 cells. Thus, circ\u20100002814 may be considered as a potential diagnostic marker and therapeutic target for preeclampsia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15297","type":"journal-article","created":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T00:30:30Z","timestamp":1653870630000},"page":"1698-1709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Circ\u20100002814 participates in proliferation and migration through miR\u2010210 and FUS\/VEGF pathway of preeclampsia"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3118-0713","authenticated-orcid":false,"given":"Na","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Guizhou Provincial People's Hospital  Guiyang Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbi","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, 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