{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:30:55Z","timestamp":1787697055127,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T00:00:00Z","timestamp":1655510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Preeclampsia (PE), the most significant adverse exposure to cardiovascular risk during pregnancy, is one of the three major factors contributing to maternal and fetal mortality and the leading cause of preterm birth. Recently, various miRNAs have been reported to participate in PE occurrence and development. Nevertheless, the regulatory impact of miR\u2010195\u20105p in PE is still indistinct.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative realtime\u2010PCR (qRT\u2010PCR), western blot, and fluorescence in situ hybridization (FISH) assay were performed to examine miR\u2010195\u20105p and FGF2 expressions in PE serum samples or HTR\u20108\/SVneo and TEV\u20101 cells. CCK8, flow cytometry, wound scratch, and transwell assays were conducted to determine cell viability, cycle, apoptosis, migration, and invasion. Dual\u2010luciferase reporter assay unveiled the relationship between miR\u2010195\u20105p and FGF2. Migration\u2010related and invasion\u2010related protein expressions were measured by western blot assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>miR\u2010195\u20105p was prominently downregulated while FGF2 was increased in serum samples from PE patients and hypoxia\u2010treated human trophoblast cells. FGF2 was predicted as a downstream target of miR\u2010195\u20105p and targeted association was verified by dual\u2010luciferase reporter assay. Functional experiments elaborated that miR\u2010195\u20105p could facilitate trophoblast cell proliferation and metastasis but hinder cell cycle and apoptosis. Inversely, overexpressing of FGF2 could reverse the effects of miR\u2010195\u20105p on trophoblast cell growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>miR\u2010195\u20105p was decreased in PE serum samples and cell lines, serving as a potential biomarker in protecting PE exacerbation by targeting FGF2.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15298","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T07:40:05Z","timestamp":1655538005000},"page":"2122-2133","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>MiR<\/scp>\u2010195\u20105p facilitates the proliferation, migration, and invasion of human trophoblast cells by targeting <scp>FGF2<\/scp>"],"prefix":"10.1111","volume":"48","author":[{"given":"Dachun","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics Nantong Maternal and Child Health 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