{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:33:07Z","timestamp":1792809187851,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T00:00:00Z","timestamp":1672012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370693"],"award-info":[{"award-number":["81370693"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Progesterone resistance is an epigenetic factor that reduces endometrial receptivity and causes implantation failure in women with endometriosis. In addition, dysregulated miRNAs contribute to the underlying pathogenic mechanisms of endometriosis. This study aimed to determine the effect of miR\u2010297 on the progesterone receptor (PR) expression and on insufficient decidualization of endometrial stromal cells (ESCs) within the eutopic endometria of infertile women with minimal or mild endometriosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ESCs were isolated from infertile endometriosis and normal patients and were transfected with miR\u2010297 mimic or miR\u2010297 inhibitor or respective control. qRT\u2010PCR and western blot were conducted to quantify the expression of miR\u2010297 and PR. The effect of miR\u2010297 on ESCs decidualization was investigated by induced decidualization in vitro<jats:italic>.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed an increase in miR\u2010297 expression and a decrease in the expression of PR in the ESCs from endometriosis patients. Moreover, the expression of PR, most notably PRB, was found to be downregulated following transfection with miR\u2010297 mimic and upregulated following treatment with miR\u2010297 inhibitor. In addition, overexpressed miR\u2010297 inhibited the decidualization of ESCs in vitro<jats:italic>.<\/jats:italic> We further determined that miR\u2010297 exerts direct regulatory effects on PR expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We demonstrated that miR\u2010297 interferes with fertility by repressing the expression of PR and preventing efficient decidualization in eutopic endometria. Further, miR\u2010297 directly contributes to progesterone resistance in minimal or mild cases of endometriosis. Thus, regulation of miR\u2010297 may prove to be a promising therapeutic strategy for endometriosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15526","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T03:19:35Z","timestamp":1672111175000},"page":"956-965","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>miR<\/scp>\u2010297 inhibits expression of progesterone receptor and decidualization in eutopic endometria of endometriosis"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7060-0883","authenticated-orcid":false,"given":"Tingting","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The People's Hospital of Leshan  Leshan China"},{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjiao","family":"Pei","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunwei","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9306-588X","authenticated-orcid":false,"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology West China Second University Hospital of Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17403-5"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"68","article-title":"High prevalence of endometriosis in infertile women with normal ovulation and normospermic partners","volume":"92","author":"Christel M","year":"2008","journal-title":"Fertil Steril"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8545(05)70302-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)48429-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.04.067"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/6.1.56"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-991044"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1389.036"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/GCO.0b013e3283630d94"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1691"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu056"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2741\/E669"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.01.113"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.67.393"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719111415546"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151651"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.03.013"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2008-0387"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmp034"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew223"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018-00374"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.78.3.642"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2017.03.013"},{"key":"e_1_2_9_25_1","first-page":"252","article-title":"Status of current research on endometriosis","volume":"43","author":"Giudice LC","year":"1998","journal-title":"J Reprod Med"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1586\/eci.11.53"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199707243370401"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gam063"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gap068"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12288"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.03.006"},{"issue":"10","key":"e_1_2_9_32_1","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1210\/mend.7.10.8123133","article-title":"Antagonist\u2010occupied human progesterone B\u2010receptors activate transcription without binding to progesterone response elements and are dominantly inhibited by A\u2010receptors","volume":"7","author":"Tung L","year":"1993","journal-title":"Mol Endocrinol"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.9.3102-3115.2000"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1780357"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.15526","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jog.15526","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.15526","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T13:03:04Z","timestamp":1692277384000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.15526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,26]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1111\/jog.15526"],"URL":"https:\/\/doi.org\/10.1111\/jog.15526","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"value":"1341-8076","type":"print"},{"value":"1447-0756","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,26]]},"assertion":[{"value":"2022-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}