{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T21:48:29Z","timestamp":1782337709573,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T00:00:00Z","timestamp":1689638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Spontaneous abortion (SA) is a multiple\u2010original syndrome with immune imbalance as one of its major risk factors. As Wharton's jelly\u2010mesenchymal stem cells (WJ\u2010MSCs) are considered to be able to prevent abortion, this study aims to explore the currently poorly understood underlining molecular signaling pathways and regulatory mechanisms of WJ\u2010MSCs in pregnancy maintenance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Abortion mode is established by subcutaneous injection of bromocriptine in rat on day 9 and abortion prevention is achieved by WJ\u2010MSCs injection via tail vein. WJ\u2010MSCs were cultured with\/without the inhibitors of JAK\/STAT or NF\u2010\u03baB. The uterus was collected on the 14th day of gestation and the rate of embryo absorption was calculated. The expression of Th1\/Th2\/Th3 cytokines in decidual, placental tissue, and peripheral blood was analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>WJ\u2010MSCs treatment significantly reduced the abortion rate in bromocriptine\u2010treated pregnancy such that it was not significantly different from a normal pregnancy. JAK\/STAT inhibition abolished pregnancy preserving effects of WJ\u2010MSCs but NF\u2010\u03baB inhibition did not. The levels of Th1\u2010related cytokines and mRNA levels in the bromocriptine abortion model were significantly higher than the normal pregnancy group and ethanol control group, while levels of the Th2\u2010related cytokines and mRNA levels significantly decreased. WJ\u2010MSCs transfusion into the abortion model restored cytokine profiles such that they were not significantly different from the normal pregnancy group and ethanol control group. JAK\/STAT inhibition of WJ\u2010MSCs prevented their effect on cytokine and mRNA levels, but NF\u2010\u03baB inhibition did not.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>WJ\u2010MSCs significantly lower the rate of embryo resorption of spontaneous abortion by reducing Th1\u2010related cytokines while increasing Th2 and Th3\u2010related cytokines in JAK\/STAT\u2010dependent manner.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15748","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T06:36:54Z","timestamp":1689748614000},"page":"2417-2426","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Human Wharton's jelly\u2010derived mesenchymal stem cells prevent pregnancy loss in a rat by <scp>JAK<\/scp>\/<scp>STAT<\/scp>\u2010mediated immunomodulation"],"prefix":"10.1111","volume":"49","author":[{"given":"Xiaoying","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Affiliated Hospital of Nantong University  Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongrong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Yancheng Third People's Hospital  Yancheng Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Affiliated Hospital of Nantong University  Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Affiliated Hospital of Nantong University  Nantong Jiangsu 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