{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T05:19:51Z","timestamp":1770009591774,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960278"],"award-info":[{"award-number":["81960278"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Most endometrial cancer (EC) patients are diagnosed at an early\u2010stage (FIGO stage I or II), with a favorable prognosis. However, some high\u2010grade, early\u2010stage EC patients have unexpected recurrences and undesirable results, the molecular alterations that underlie these tumors are far from being fully understood. Our goal was to use proteome analysis to examine dysregulated pathways in this specific subgroup of EC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used data\u2010independent acquisition (DIA) quantitative proteomics to analyze cancer and matched paracancerous tissues from 20 EC patients (10 high\u2010grade and 10 low\u2010grade). Immunohistochemistry (IHC) analysis was used to validate protein expression of six hub genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 7107 proteins were quantified, while 225 downregulated and 366 upregulated proteins in high\u2010grade cancer tissues, 130 downregulated and 413 upregulated proteins in high\u2010grade paracancerous tissues. The pathway associated with oxidative phosphorylation (OXPHOS) was upregulated and have similar expression patterns in high\u2010grade EC tissues and matched paracancerous tissues. OXPHOS\u2010related protein, ATP5F1D showed the best classification and diagnostic ability in distinguishing high\u2010grade from low\u2010grade EC. In both cancer and paracancerous tissues, double\u2010label immunofluorescence demonstrated ITGA4 and COL4A1 co\u2010localized at the basal membrane.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our present works elucidate that metabolism reprogramming is associated with high\u2010grade, early\u2010stage EC, particularly OXPHOS is upregulated. Noticeably, the paracancerous tissues have undergone molecular changes similar to cancer tissues, maybe they have signal exchange via secreted proteins (ITGA4 and COL4A1). The upregulation of OXPHOS\u2010related proteins may be the potential biomarker for EC diagnosis, and targeting OXPHOS metabolism might be an effective treatment for high\u2010grade, early\u2010stage EC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.15834","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T22:04:11Z","timestamp":1700604251000},"page":"233-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Data\u2010independent acquisition for proteomic applications in <scp>early<\/scp>\u2010<scp>stage<\/scp> endometrial cancer progression"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-9997","authenticated-orcid":false,"given":"Yuemei","family":"Cheng","sequence":"first","affiliation":[{"name":"The First Clinical Medical College of Lanzhou University Department of Obstetrics and Gynecology, Gansu Provincial Clinical Research Center for 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