{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:14:25Z","timestamp":1787015665761,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Recurrent pregnancy loss (RPL) has a multifactorial etiology, with a majority of cases remaining unexplained. To account for these unexplained cases, possible male factors are being explored. Conventional semen analysis lacks a qualitative assessment of sperms and information regarding sperm DNA integrity. Sperm DNA fragmentation (SDF) has diagnostic value in unexplained RPL, and it may account for a number of unexplained cases. Hence, we planned a study to explore and evaluate the impact of sperm DNA fragmentation in couples with unexplained recurrent pregnancy losses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>\n                      Analytical cross\u2010sectional study was conducted at a tertiary\u2010level referral facility in India between August 2021 and July 2023. Participants (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200970) were divided into two groups\u2014male partners of couples with unexplained RPL (following spontaneous conceptions) (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200935) and men with at least one previous live birth (spontaneous or following fertility treatments for female factor infertility such as ovulation induction or intrauterine insemination) as controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200935). Neither of the two groups of couples recruited for this study had undergone ART as fertility treatment. Primary outcome assessed was mean DNA fragmentation index (DFI). Secondary outcomes included differences in semen parameters such as sperm concentration, progressive sperm motility and morphology, proportion of men with high (\u226530%) and low DFI in the two groups, and the association between various semen parameters and DFI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Univariate logistic regression revealed that sperm DNA fragmentation was higher in men with unexplained RPL (30.0; IQR (interquartile range) 19.0, 46.0) as compared to controls (22.0; IQR 14.0, 30.0) although it was not statistically significant (OR, odds ratio, 1.02; 95% CI 1.0\u20131.1,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.08). A higher proportion of men with unexplained RPL had DFI \u226530% compared to controls (54.2% vs. 25.7%; OR 3.43 (95% CI 1.2\u20139.4);\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02). No statistically significant differences were observed in semen volume, sperm concentration, progressive motility, and morphology between the two groups. Sperm DNA fragmentation index also showed a weak but significant inverse relationship with sperm morphology (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.336,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.004).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The current study did not show any significant difference in the mean sperm DNA fragmentation levels in male partners of couples with unexplained RPL compared to controls. However, a higher proportion of men with DFI \u226530% were observed in unexplained RPL population when compared to controls.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.16041","type":"journal-article","created":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T03:35:03Z","timestamp":1722656103000},"page":"1687-1696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Impact of sperm\n                    <scp>DNA<\/scp>\n                    fragmentation in couples with unexplained recurrent pregnancy loss: A cross\u2010sectional study"],"prefix":"10.1111","volume":"50","author":[{"given":"Megha","family":"Solanki","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine and Surgery Christian Medical College  Vellore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1952-9364","authenticated-orcid":false,"given":"Treasa","family":"Joseph","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine and Surgery Christian Medical College and Hospital  Vellore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Muthukumar","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine and Surgery Christian Medical College and Hospital  Vellore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasanna","family":"Samuel","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Christian Medical College  Vellore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6506-0133","authenticated-orcid":false,"given":"T. K.","family":"Aleyamma","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine and Surgery Christian Medical College and Hospital  Vellore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0316-636X","authenticated-orcid":false,"given":"Mohan S.","family":"Kamath","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine and Surgery Christian Medical College  Vellore India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","unstructured":"ESHRE guideline: recurrent pregnancy loss: an update in 2022\u2020 | Human Reproduction Open | Oxford Academic [Internet]. [cited 2023 Sep 3]. Available from:https:\/\/academic.oup.com\/hropen\/article\/2023\/1\/hoad002\/7068067","DOI":"10.1093\/hropen\/hoad002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-11-154"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2021.08.037"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32989-1"},{"key":"e_1_2_11_6_1","doi-asserted-by":"crossref","unstructured":"Antioxidants for male subfertility\u2014de Ligny W\u20142022 | Cochrane Library [Internet]. [cited 2023 Aug 15]. 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