{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:23:03Z","timestamp":1790947383096,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,10]],"date-time":"2024-11-10T00:00:00Z","timestamp":1731196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Research on the effects of methylphenidate on female fertility is limited. This study evaluated the effects of methylphenidate on reproductive function, oxidants, antioxidants, proinflammatory cytokines, prolactin, and cortisol in female rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010eight albino Wistar female rats were divided into four groups consisting of 12 rats, which were given pure water orally once daily for 7\u2009days (HG\u20101), 10 mg\/kg methylphenidate orally once daily for 7\u2009days (MP\u20101), pure water orally once daily for 30\u2009days (HG\u20102), and 10 mg\/kg methylphenidate orally once daily for 30\u2009days (MP\u20102). At the end of the treatment periods, tail vein blood was collected from six rats per group for prolactin and cortisol determination. Subsequently, euthanasia was performed and the ovaries were removed. Ovaries were analyzed for malondialdehyde (MDA), total glutathione (tGSH), superoxide dismutase (SOD), catalase (CAT), interleukin\u20101 beta (IL\u20101\u03b2), and tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), and immunohistochemically. For breeding, the remaining six rats were mated with male rats for 1\u2009month. Rats that failed to give birth were classed as infertile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A comparison of MP\u20101 and MP\u20102 groups to healthy controls revealed an elevation in MDA and corticosterone levels, and a decline in tGSH, SOD, and CAT levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Methylphenidate did not affect prolactin, IL\u20101\u03b2, and TNF\u2010\u03b1 levels (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). MP\u20101 and MP\u20102 exhibited immunopositivity for 8\u2010hydroxy\u20102'\u2010deoxyguanosine (8\u2010OHDG). MP\u20102 rats developed 66.7% infertility while MP\u20101, HG\u20101, and HG\u20102 rats did not.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In ovaries, methylphenidate caused oxidative stress, but did not induce inflammation. Long\u2010term use of methylphenidate caused increased cortisol levels and infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.16145","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T07:59:47Z","timestamp":1731311987000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The effect of methylphenidate on the reproductive function of female rats"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0983-3204","authenticated-orcid":false,"given":"Numan","family":"Cim","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Istanbul Haseki Training and Research Hospital Republic of Turkey Ministry of Health  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2512-7705","authenticated-orcid":false,"given":"Bunyamin","family":"Cim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Islahiye State Hospital Republic of Turkey Ministry of Health  Gaziantep Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9313-4056","authenticated-orcid":false,"given":"Emine Fusun","family":"Akyuz Cim","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Florence Nightingale Hospital, Medical Faculty Demiroglu Bilim University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4992-1241","authenticated-orcid":false,"given":"Seval","family":"Bulut","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Erzincan Binali Yildirim University  Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renad","family":"Mammadov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Erzincan Binali Yildirim University  Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahad\u0131r","family":"Suleyman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Erzincan Binali Yildirim University  Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurinisa","family":"Yucel","sequence":"additional","affiliation":[{"name":"Pharmacy Services Program, Vocational School of Health Services Erzincan Binali Yildirim University  Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Sefa","family":"Mendil","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1711-5499","authenticated-orcid":false,"given":"Taha Abdulkadir","family":"Coban","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Erzincan Binali Yildirim University  Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9239-4099","authenticated-orcid":false,"given":"Halis","family":"Suleyman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Erzincan Binali Yildirim University  Erzincan Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,10]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"VergheseC AbdijadidS.Methylphenidate. 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