{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:27:39Z","timestamp":1753878459148,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012021","name":"Iran University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the efficacy of a microfluidic culture system supplemented with follicular fluid meiosis\u2010activating sterol (FF\u2010MAS) on the maturation of immature oocytes in patients with polycystic ovarian syndrome (PCOS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 438 germinal vesicle oocytes from 163 PCOS patients were included. Oocytes were divided into five groups: (1) cultured in static drops without FF\u2010MAS, (2) cultured in static drops with FF\u2010MAS, (3) cultured in a microfluidic device without FF\u2010MAS, (4) cultured in a microfluidic device with FF\u2010MAS for the first 2\u2009h, and (5) cultured in a microfluidic device with FF\u2010MAS for 24\u2009h. Maturation, fertilization, and embryo development rates were assessed, alongside gene expression analysis using quantitative real\u2010time PCR. Ultrastructural analysis was performed using transmission electron microscopy (TEM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The maturation rates for oocytes in groups 1 to 5 were 36%, 48%, 42%, 64%, and 65%, respectively, with groups 4 and 5 showing significant increases (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Fertilization rates were higher in groups 4 and 5 (p\u2009&lt;\u20090.05). High\u2010quality embryo formation rates were significantly higher in groups 3, 4, and 5. Gene expression analysis indicated enhanced expression of BRCA1 and TP53 in the dynamic culture groups. Ultrastructural analysis showed improved mitochondrial integrity and cytoplasmic maturation in the dynamic groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The dynamic microfluidic culture system with FF\u2010MAS supplementation significantly enhances oocyte maturation and embryo quality in PCOS patients by closely mimicking the natural in vivo environment. This approach shows promise for optimizing in vitro oocyte maturation and improving fertility outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.16203","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T07:49:23Z","timestamp":1737532163000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing oocyte competence in patients with polycystic ovarian syndrome: A microfluidic study on the effects of follicular fluid meiosis\u2010activating sterol in in vitro oocyte maturation"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9991-8019","authenticated-orcid":false,"given":"Hossein","family":"Torkashvand","sequence":"first","affiliation":[{"name":"Department of Anatomy, School of Medicine Iran University of Medical Sciences  Tehran Iran"},{"name":"Fertility and Infertility Research Center Hamadan University of Medical Sciences  Hamadan 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