{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:26:45Z","timestamp":1788805605669,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960284"],"award-info":[{"award-number":["81960284"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Preeclampsia (PE) is a pregnancy\u2010specific hypertensive disorder and one of the leading causes of maternal mortality. However, its etiology and pathogenesis are not yet fully clarified. This study aimed to uncover methylation\u2010regulated oxidative stress\u2010related placenta\u2010specific molecules in PE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two PE datasets, GSE57767 and GSE25906, were subjected into this study. The oxidative stress\u2010related genes were derived from GeneCards database. Differential methylation and expression analysis were applied to identify methylation\u2010regulated oxidative stress\u2010related genes in PE. The methylation\u2010regulated oxidative stress\u2010related placenta\u2010specific molecules were determined by receiver operating characteristic (ROC) analysis. The single\u2010gene gene set enrichment analysis (GSEA) were executed using the R \u201cclusterProfiler.\u201d The transcription factor (TF)\u2010gene regulatory network of placenta\u2010specific molecules was created through Network Analyst database and Cytoscape software. The drug\u2010gene network of placenta\u2010specific molecules were developed through DGIdb database and Cytoscape software. Eventually, we further examined biomarker expression trends in the collected clinical samples using real time quantitative PCR (RT\u2010qPCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 13 methylation\u2010regulated oxidative stress\u2010related genes in PE were identified. Then, five genes (<jats:italic>VIM<\/jats:italic>, <jats:italic>SNCA<\/jats:italic>, <jats:italic>PIK3CG<\/jats:italic>, <jats:italic>DNM2<\/jats:italic>, and <jats:italic>BMP6<\/jats:italic>) were authenticated as methylation\u2010regulated oxidative stress\u2010related placenta\u2010specific molecules in PE by ROC curves, suggesting a potential clinical diagnostic value. Single\u2010gene GSEA pointed to the linkage of these five genes to the immune\u2010related pathways, ferroptosis, and oxidative phosphorylation. Finally, a TF\u2010gene regulatory network containing 32 nodes and 38 edges and a drug\u2010gene network containing 126 nodes and 123 edges were generated based on methylation\u2010regulated oxidative stress\u2010related placenta\u2010specific molecules in PE. Ultimately, the experimental results confirmed that the expression trends of <jats:italic>VIM<\/jats:italic>, <jats:italic>PIK3CG<\/jats:italic>, and <jats:italic>BMP6<\/jats:italic> in our collected clinical samples were in line with the expression trends in the GSE25906 dataset.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Three genes, <jats:italic>VIM<\/jats:italic>, <jats:italic>PIK3CG<\/jats:italic>, and <jats:italic>BMP6<\/jats:italic>, were identified as methylation\u2010regulated oxidative stress\u2010related placenta\u2010specific molecules in PE. This might have helped to understand the pathogenesis of the disease and might also have provided new perspectives on the diagnosis and treatment of PE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.16209","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T06:52:30Z","timestamp":1741589550000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrated analysis of <scp>DNA<\/scp> methylation and transcriptome profiles to identify oxidative stress\u2010related placenta\u2010specific molecules for preeclampsia"],"prefix":"10.1111","volume":"51","author":[{"given":"Yang","family":"Xu","sequence":"first","affiliation":[{"name":"Guizhou Medical University  Guizhou China"},{"name":"Guizhou Provincial People's Hospital  Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Guizhou Provincial 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