{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:59:06Z","timestamp":1783108746550,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to identify potential biomarkers in high\u2010risk human papillomavirus (HR\u2010HPV) infections and related cervical lesions through the analysis of vaginal metabolic profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and methods<\/jats:title>\n                    <jats:p>\n                      Forty cervicovaginal secretion samples were collected from healthy individuals, HR\u2010HPV\u2010positive individuals, low\u2010grade squamous intraepithelial lesion patients, high\u2010grade squamous intraepithelial lesion patients, and cervical cancer (CC) patients. All samples were analyzed using liquid chromatography\u2013mass spectrometry (LC\u2013MS). Metabolites with significant differences were identified using the variable importance in projection (VIP\u2009&gt;\u20091) derived from the orthogonal least partial squares discriminant analysis (OPLS\u2010DA) model and the\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) of the Student's\n                      <jats:italic>t<\/jats:italic>\n                      test. Utilizing hierarchical cluster analysis and receiver operating characteristic curve analysis, the optimal biomarkers were identified, and the diagnostic potential of these biomarkers was evaluated. Significantly enriched pathways of the differential metabolites were explored using metabolic pathway analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      LC\u2013MS analysis of the vaginal metabolomic profiles revealed unique molecular characteristics of different states of HR\u2010HPV infection. Based on persistent HR\u2010HPV infection, 3\u2010Isochromanone increased, and DG(2:0\/5\u2010iso PGF2VI\/0:0) and (4R,5S,7R,11x)\u201011,12\u2010Dihydroxy\u20101(10)\u2010spirovetiven\u20102\u2010one 12\u2010glucoside decreased (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) with the aggravation of cervical lesions, suggesting their potential as biomarkers to predict various stages of cervical lesions. Additionally, analysis of metabolic pathway enrichment for differential metabolites identified the phenylalanine, tyrosine, and tryptophan biosynthesis pathways as the key metabolic pathways in the progression of cervical lesions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>By measuring the metabolome of vaginal secretions, specific metabolites can effectively distinguish persistent HR\u2010HPV infections and different grades of cervical lesions, which may become an important means for the early screening and diagnosis of CC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70035","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T04:16:36Z","timestamp":1754972196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Vaginal metabolic profiling reveals biomarkers characteristics of high\u2010risk\n                    <scp>HPV<\/scp>\n                    infection and cervical lesions"],"prefix":"10.1111","volume":"51","author":[{"given":"Xiaoling","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoge","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"},{"name":"Zhengzhou Central Hospital  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6594-5373","authenticated-orcid":false,"given":"Sijing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoning","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1634-3254","authenticated-orcid":false,"given":"Ying","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical University  Chongqing 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