{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:28:12Z","timestamp":1789698492584,"version":"4.0.1"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K07957"],"award-info":[{"award-number":["23K07957"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K19278"],"award-info":[{"award-number":["24K19278"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP22fk0108540"],"award-info":[{"award-number":["JP22fk0108540"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP22fk0108612"],"award-info":[{"award-number":["JP22fk0108612"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23fk0108604"],"award-info":[{"award-number":["JP23fk0108604"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to clarify whether the detection rate of group B\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      (\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      ) in screening tests for pregnant women at 35\u201337\u2009weeks of gestation increases using the selective enrichment media available in Japan.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Vaginal\u2013rectal swabs were collected from pregnant women at 35\u201337\u2009weeks of gestation from nine obstetric medical institutions in the Nagasaki Prefecture. The collected swabs were inoculated into selective enrichment media and directly plated onto blood agar to investigate the differences in the isolation frequencies of\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We collected vaginal\u2013rectal swabs from 116 individuals and successfully isolated\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      from 22 individuals (19.0%) using a selective enrichment broth. When the swabs were directly plated onto blood agar for isolation,\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      was not isolated in nine (40.9%) out of the 22 positive samples. Among these nine samples, six (66.7%) showed reduced pigment production in at least one of the selective enrichment broths that detected\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      based on pigment production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In a\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      screening test for pregnant women, use of a selective enrichment broth resulted in an approximately 1.7\u2010fold increase in the number of positive samples compared to the direct plating method on blood agar. Although not described in the American Society for Microbiology guidelines,\n                      <jats:styled-content style=\"fixed-case\">RambaQUICK GBS<\/jats:styled-content>\n                      broth and Poamedia semisolid medium for\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      , both available in Japan, detected\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      significantly more effectively than the direct plating method on blood agar. This finding supports the utility of selective enrichment broths for the\n                      <jats:styled-content style=\"fixed-case\">GBS<\/jats:styled-content>\n                      screening of pregnant women.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70084","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T06:37:37Z","timestamp":1758782257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Increased recovery of\n                    <i>Streptococcus agalactiae<\/i>\n                    from vaginal\u2013rectal swabs using a selective enrichment broth medium"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8987-0250","authenticated-orcid":false,"given":"Satoshi","family":"Nakano","sequence":"first","affiliation":[{"name":"Antimicrobial Resistance Research Center National Institute of 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