{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T02:08:47Z","timestamp":1761271727361,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To study the function of pregnancy\u2010specific glycoprotein 1 (PSG1) in regulating proliferation, migration, and vascular tone of human umbilical vein endothelial cells (HUVECs), and further explore its role in placental development and potential molecular mechanisms through co\u2010culturing HUVECs with HTR8\/svneo cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>HUVECs were treated with 2, 4, and 8\u2009\u03bcg\/mL PSG1. The Cell Counting Kit\u20108 assay was used to test cell proliferation, apoptosis by Annexin V\/PI staining, and intracellular calcium and nitric oxide (NO) levels by fluorescence microscopy. Western blotting quantified the expression of vascular endothelial growth factor (VEGF), transforming growth factor\u2010beta (TGF\u2010\u03b2), endothelial nitric oxide synthase (eNOS), calcium release\u2010activated calcium channel protein 3 (Orai3), and PSG1. To study PSG1's effect on placental development, HUVECs were co\u2010cultured with HTR8\/svneo cells. siRNA\u2010mediated knockdown of PSG1 was performed in both cell types to evaluate its impact on endothelial function, vascular tone, and trophoblast\u2010endothelial cell interactions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PSG1 treatment enhanced HUVEC proliferation, with high concentrations reducing apoptosis. PSG1 upregulated the expression of VEGF, TGF\u2010\u03b2, eNOS, and Orai3, and significantly increased both intracellular calcium and NO levels. Knockdown of PSG1 reduced these effects in HUVECs, and co\u2010culture with PSG1\u2010deficient trophoblast cells further diminished HUVEC proliferation, enhanced apoptosis, and decreased NO release. Our study highlighted the crucial role of PSG1 in endothelial cell function and vascular tone regulation. Furthermore, PSG1 modulation influenced eNOS activity, enhancing NO release, which contributed to vascular dilation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>PSG1 promotes HUVEC proliferation, inhibits apoptosis, and regulates vascular tone through the TGF\u2010\u03b2\/Orai3 signaling pathway, primarily by modulating NO production.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70092","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:11:21Z","timestamp":1760058681000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>PSG1<\/scp>\n                    in Regulating Proliferation and Migration of Human Umbilical Vein Endothelial Cells Through the\n                    <scp>TGF<\/scp>\n                    \u2010\u03b2\/Orai3 Signaling Pathway"],"prefix":"10.1111","volume":"51","author":[{"given":"Yali","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"},{"name":"Department of Obstetric and Gynecology Jinhua Central Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaofang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanyan","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mianbo","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lichao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"},{"name":"Department of Obstetric and Gynecology Jinhua Central Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilan","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3683-4874","authenticated-orcid":false,"given":"Xufei","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Obstetric and Gynecology Jinhua Maternal and Child Health Care Hospital  Jinhua Zhejiang China"},{"name":"Department of Obstetric and Gynecology Jinhua Central Hospital  Jinhua Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,9]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.53"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.082412"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-20-0169"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3131"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-024-00526-w"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01460-1"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/157016112798829760"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.2741\/3828"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2023.06.014"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01430-7"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15163564"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/advances\/nmab070"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.01.004"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200105)31:5<1417::AID-IMMU1417>3.0.CO;2-C"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.307123"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9061995"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.082412"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.098251"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01924"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-669"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00005.2002"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12504"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2009.10.007"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2016.02.004"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.6.2.11686"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00268-6"},{"key":"e_1_2_13_28_1","first-page":"3","article-title":"Pregnancy\u2010Specific 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