{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T06:05:22Z","timestamp":1764137122235,"version":"3.46.0"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T00:00:00Z","timestamp":1762819200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to investigate the clinical significance of actinin\u20104 in endometrial carcinoma. Actinin\u20104, an actin\u2010binding protein involved in cytoskeletal dynamics, has been implicated in the progression of various cancers; however, its precise role in endometrial carcinoma is not fully understood. This research sought to evaluate actinin\u20104 protein expression and gene amplification and correlate these findings with clinicopathological parameters and patient survival to determine its prognostic value.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A retrospective analysis was conducted on endometrial carcinoma patients who underwent surgical resection. Actinin\u20104 protein expression was assessed using immunohistochemical staining (IHC), and\n                      <jats:italic>ACTN4<\/jats:italic>\n                      gene amplification was evaluated by fluorescence in\u00a0situ hybridization (FISH). The intensity of actinin\u20104 staining was graded, and gene amplification of\n                      <jats:italic>ACTN4<\/jats:italic>\n                      was defined using the\n                      <jats:italic>ACTN4<\/jats:italic>\n                      \/\n                      <jats:italic>CEP19<\/jats:italic>\n                      ratio. Statistical analysis, including Kaplan\u2013Meier survival analysis and Cox proportional hazards modeling, was performed to correlate actinin\u20104 expression with clinicopathological features and survival outcomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overexpression of actinin\u20104 protein by IHC was significantly associated with advanced clinical stage and histological subtypes. While no significant difference was observed in overall survival (OS), patients with high actinin\u20104 IHC demonstrated significantly poorer progression\u2010free survival (PFS).\n                      <jats:italic>ACTN4<\/jats:italic>\n                      gene amplification by FISH was significantly associated with poorer prognosis for both OS and PFS compared to the group without amplification.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study suggests that actinin\u20104 plays a role in the progression of endometrial carcinoma, particularly influencing tumor aggressiveness and progression\u2010free survival.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70132","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T23:40:24Z","timestamp":1762904424000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prognostic Significance of Actinin\u20104 Protein Expression and Gene Amplification in Endometrial Carcinoma"],"prefix":"10.1111","volume":"51","author":[{"given":"Li","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6827-601X","authenticated-orcid":false,"given":"Yutaka","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Molecular Prevention, Institute for Advanced Medical Science Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8199-9917","authenticated-orcid":false,"given":"Masafumi","family":"Toyoshima","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0913-8279","authenticated-orcid":false,"given":"Mika","family":"Terasaki","sequence":"additional","affiliation":[{"name":"Department of Analytic Human Pathology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9158-4897","authenticated-orcid":false,"given":"Akihito","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Analytic Human Pathology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3996-2624","authenticated-orcid":false,"given":"Shunji","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazufumi","family":"Honda","sequence":"additional","affiliation":[{"name":"Department of Molecular Prevention, Institute for Advanced Medical Science Nippon Medical School  Tokyo Japan"},{"name":"Department of Molecular Prevention, Graduate School of Medicine Nippon Medical School  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1514010"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010024\u201000934\u20107"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12113"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2015.190"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.6.1383"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.10.004"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04163.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt293"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.623"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1272\/jnms.JNMS.2022_89-118"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1272\/jnms.JNMS.2024_91-216"},{"key":"e_1_2_10_14_1","first-page":"1250","volume-title":"Brek & Novak's Gynecology","author":"Lurain J. 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