{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T16:16:44Z","timestamp":1781713004085,"version":"3.54.5"},"reference-count":76,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020374","name":"Ministry of AYUSH, Government of India","doi-asserted-by":"publisher","award":["TECH\u201021013\/8\/2024\u2010CCRUM\u2010HQ"],"award-info":[{"award-number":["TECH\u201021013\/8\/2024\u2010CCRUM\u2010HQ"]}],"id":[{"id":"10.13039\/100020374","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      The present study aimed to evaluate the therapeutic potential of the Unani formulation\n                      <jats:italic>Ark\u2010e\u2010Kasni<\/jats:italic>\n                      in alleviating the symptoms of polycystic ovary syndrome (PCOS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In the present study, letrozole and a high\u2010fat diet (HFD) were used to induce PCOS\u2010like symptoms in rats. After induction, different groups were treated with varying doses of\n                      <jats:italic>Ark\u2010e\u2010Kasni<\/jats:italic>\n                      (5, 10, and 20\u2009mL\/kg), metformin, and finasteride. At the end of treatment, blood and ovaries were collected for biochemical and histological analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A significant weight gain and prolonged diestrous phase were observed in PCOS rats, accompanied by a considerable elevation in LH (42.52\u2009\u00b1\u20090.90 mIU\/mL) and testosterone (8370\u2009\u00b1\u2009122.18\u2009pg\/mL), and a low level of FSH (11.86\u2009\u00b1\u20090.43\u2009mIU\/mL). Moreover, an increase in insulin (14.31\u2009\u00b1\u20090.35 mIU\/mL), TNF\u2010\u03b1 (358.81\u2009\u00b1\u20099.81\u2009pg\/mL), IL\u20101\u03b2 (143.74\u2009\u00b1\u20093.39\u2009pg\/mL), and IL\u20106 (154.04\u2009\u00b1\u20092.04\u2009pg\/mL), and a reduction in SOD, CAT, and GSH were noticed. While the\n                      <jats:italic>Ark\u2010e\u2010Kasni<\/jats:italic>\n                      restored the levels of LH (30.97\u2009\u00b1\u20091.04\u2009mIU\/mL), testosterone (5651.83\u2009\u00b1\u2009182.69\u2009pg\/mL), FSH (15.66\u2009\u00b1\u20090.56 mIU\/mL), insulin (5.48\u2009\u00b1\u20090.23 mIU\/mL), TNF\u2010\u03b1 (212.16\u2009\u00b1\u20098.66\u2009pg\/mL), IL\u20101\u03b2 (78.79\u2009\u00b1\u20091.46\u2009pg\/mL), and IL\u20106 (74.53\u2009\u00b1\u20091.60\u2009pg\/mL), and lipid markers. Histological and microscopic analysis showed reduced cystic follicles and enhanced corpus luteum formation, improving ovarian morphology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The findings suggest that\n                      <jats:italic>Ark\u2010e\u2010Kasni<\/jats:italic>\n                      ameliorates PCOS through diverse molecular mechanisms.\n                      <jats:italic>Ark\u2010e\u2010Kasni<\/jats:italic>\n                      may modulate steroidogenic enzyme activity to reduce hyperandrogenism, enhance insulin sensitivity through the PI3K\/Akt pathway, and inhibit the downstream inflammatory pathway of NF\u2010\u03baB.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70228","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:20:18Z","timestamp":1773102018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Protective Effect of \u201c\n                    <i>Ark\u2010e\u2010Kasni<\/i>\n                    ,\u201d a Unani Formulation, in Letrozole and High\u2010Fat Diet\u2013Induced Polycystic Ovarian Syndrome in Rats"],"prefix":"10.1111","volume":"52","author":[{"given":"Kamini R.","family":"Shirasath","sequence":"first","affiliation":[{"name":"Department of Pharmacology Shri Vile Parle Kelavani Mandal's Institute of Pharmacy  Dhule Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Karwasra","sequence":"additional","affiliation":[{"name":"Central Council for Research in Unani Medicine (CCRUM)  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. Zaheer","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Central Council for Research in Unani Medicine (CCRUM)  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawan","family":"Kumar","sequence":"additional","affiliation":[{"name":"Central Council for Research in Unani Medicine (CCRUM)  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah","family":"Alam","sequence":"additional","affiliation":[{"name":"Regional Research Institute of Unani Medicine (RRIUM)  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameer N.","family":"Goyal","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Shri Vile Parle Kelavani Mandal's Institute of Pharmacy  Dhule Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3293-5607","authenticated-orcid":false,"given":"Yogeeta O.","family":"Agrawal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics Shri Vile Parle Kelavani Mandal's Institute of Pharmacy  Dhule Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.838204"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252011146"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2025.156245"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12902\u2010022\u201001208\u2010y"},{"issue":"8","key":"e_1_2_13_6_1","first-page":"671","article-title":"Drug Treatments for Polycystic Ovary Syndrome","volume":"79","author":"Radosh L.","year":"2009","journal-title":"American Family Physician"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1294406"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/jdds.jdds_33_21"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.22270\/jddt.v13i6.6092"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050541d"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prenap.2025.100397"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.33545\/26646536.2024.v6.i1a.51"},{"issue":"1","key":"e_1_2_13_13_1","first-page":"63","article-title":"Resolution of Polycystic Ovaries by Unani Formulation: A Case Report","volume":"6","author":"Riaz S.","year":"2022","journal-title":"International Journal of AYUSH Case Reports"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ipha.2023.08.011"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.11.028"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.107043"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2014.11.016"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12041096"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16768"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201026210\u20100"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.03.023"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1457467"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathophysiology31040041"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/142535"},{"issue":"2","key":"e_1_2_13_25_1","first-page":"99","article-title":"Prenatal Kisspeptin Antagonist Exposure Prevents Polycystic Ovary Syndrome Development in Prenatally\u2010Androgenized Rats in Adulthood: An Experimental Study","volume":"21","author":"Bakhi E. 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