{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:51:52Z","timestamp":1776905512785,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the mechanism by which Atractylenolide III (ATIII) alleviates ovariectomy\u2010induced, estrogen\u2010deficient lipid metabolism disorders through the repair of intestinal inflammation and the barrier microenvironment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Female C57BL\/six mice (8\u2009weeks old) were randomly assigned to three groups: a blank control group (Con,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910), a sham surgery group (Sham,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910), and an ovariectomized (OVX) group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200970). The OVX group was further subdivided into a model group (OVX\u2009+\u2009HFD), low\u2010 and high\u2010dose ATIII groups (ATIII\u2010L, ATIII\u2010H), an estradiol (E2) group, and groups receiving fecal microbiota transplantation (FMT) from the blank control, model, or high\u2010dose ATIII donors. After 60\u2009days on a high\u2010calorie diet, treatments were administered for 28 consecutive days. Serum, liver, and intestinal tissues, and cecal contents were collected from six randomly selected mice per group. Body weight was monitored; hepatic and colonic morphology was assessed by H&amp;E staining; serum lipid profiles were determined using an automated biochemical analyzer; ELISA quantified estradiol and inflammatory cytokine levels; expression of colonic barrier\u2010related proteins was evaluated by Western blot; and gut microbiota composition was analyzed via 16S rRNA sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Under conditions of estrogen deficiency, a high\u2010calorie diet mimicking modern human intake predisposed mice to significant weight gain (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and dyslipidemia, accompanied by a spectrum of pathological alterations including intestinal barrier dysfunction (evidenced by downregulated tight junction proteins), systemic inflammation (reflected by elevated pro\u2010inflammatory cytokines), hepatic steatosis, colonic inflammatory damage, and gut microbiota dysbiosis. ATIII intervention effectively mitigated these abnormalities, as demonstrated by reduced body weight, improved lipid profiles, repaired hepatic and colonic injuries, upregulated intestinal barrier proteins, downregulated inflammatory cytokines, a tendency toward elevated estrogen levels, and enhanced gut microbial diversity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ATIII ameliorates ovariectomy\u2010induced estrogen\u2010deficient dyslipidemia by repairing intestinal barrier function and modulating intestinal inflammation. Concurrently, it exerts beneficial effects on estrogen levels and gut microbiota composition, in which the gut microbiota plays a mediating role. The experiment demonstrated that the active ingredients of traditional Chinese medicine hold significant value in treating lipid metabolism disorders in perimenopausal women, and there is potential for further in\u2010depth research into the mechanism by which they enhance efficacy through modulating gut microbiota.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70272","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:57:18Z","timestamp":1776401838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Atractylenolide\n                    <scp>III<\/scp>\n                    Alleviates the Lipid Metabolic Disorders\u00a0in Ovariectomy\u2010Induced Estrogen\u2010Deficient Mice Through Repairing Intestinal Inflammation and Microenvironment"],"prefix":"10.1111","volume":"52","author":[{"given":"Xu","family":"Yanyan","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery The Second Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9789-0905","authenticated-orcid":false,"given":"Qu","family":"Yanyu","sequence":"additional","affiliation":[{"name":"Nanjing University of Chinese Medicine  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geng","family":"Qiao","sequence":"additional","affiliation":[{"name":"Nanjing University of Chinese Medicine  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gu","family":"Jiabo","sequence":"additional","affiliation":[{"name":"Department of Anorectal Surgery The Second Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing Jiangsu 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