{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:48:31Z","timestamp":1782186511889,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gestational diabetes mellitus (GDM) is a common pregnancy\u2010related condition that poses risks to both the mother and the fetus. Although dysregulated thrombospondin\u20104 (TSP\u20104) has been observed in women with GDM, the precise role of TSP\u20104 in the pathogenesis of GDM remains unclear. This study investigates the role of TSP\u20104 in regulating trophoblast function under high glucose (HG) conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>HTR\u20108\/SVneo cells were exposed to HG to establish an in\u00a0vitro GDM model. TSP\u20104 expression was silenced via lentiviral transfection. The effects of TSP\u20104 knockdown on cell proliferation, migration, invasion, inflammation, and the PI3K\/Akt\/mTOR signaling pathway were assessed using Western blotting, Cell Counting Kit\u20108 assay, glucose uptake assay, enzyme\u2010linked immunosorbent assay (ELISA), and Transwell assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HG exposure significantly upregulated TSP\u20104 expression in HTR\u20108\/SVneo cells. TSP\u20104 knockdown under HG conditions enhanced cell migration, invasion, proliferation, and glucose uptake. Furthermore, TSP\u20104 knockdown inhibited the inflammatory response in HG\u2010treated HTR\u20108\/SVneo cells in the HG group. Notably, TSP\u20104 knockdown also modulated the activation of the PI3K\/Akt\/mTOR signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>TSP\u20104 knockdown promotes proliferation, migration, glucose uptake, reduces inflammation, and regulates the expression of the PI3K\/Akt\/mTOR signaling pathway in trophoblast cell lines under HG conditions, suggesting a potential role for TSP\u20104 in the pathophysiology of GDM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70364","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T01:27:29Z","timestamp":1781486849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Knockdown of\n                    <scp>TSP<\/scp>\n                    \u20104 Promotes Proliferation, Migration, Glucose Uptake, and Inhibits Inflammatory Damage in Trophoblast Cell Lines Exposed to High Glucose"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0049-9055","authenticated-orcid":false,"given":"Lu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Women's Hospital School of Medicine Zhejiang University of Jilin Hospital (Changchun Obstetrics and Gynecology Hospital)  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Jilin Province FAW General Hospital  Changchun Jilin 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