{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T06:01:23Z","timestamp":1784700083810,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007787","name":"National Research Centre","doi-asserted-by":"publisher","award":["16\u2010251"],"award-info":[{"award-number":["16\u2010251"]}],"id":[{"id":"10.13039\/100007787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Neural tube defects (NTDs) are common congenital anomalies of the central nervous system and are associated with substantial fetal and neonatal morbidity and mortality. Although maternal serum alpha\u2010fetoprotein (MSAFP) and ultrasonography (US) are routinely used for prenatal screening, small or atypical lesions may be missed. Circulating microRNAs have emerged as promising noninvasive biomarkers in prenatal diagnosis.<\/jats:p>\n <\/jats:sec>\n \n Aim<\/jats:title>\n To calibrate the diagnostic utility of circulating microRNA\u2010720 and microRNA\u20101275 for prenatal detection of fetal NTDs and to compare their performance with MSAFP and two\u2010 and three\u2010dimensional US.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n This case\u2013control study included 48 pregnant women undergoing second\u2010trimester screening (15\u201322\u2009weeks of gestation), divided into 24 cases with fetal NTDs and 24 controls with normal fetuses. All participants underwent detailed two\u2010 and three\u2010dimensional US evaluation and MSAFP measurement. Circulating microRNA\u2010720 and microRNA\u20101275 expression levels were quantified using real\u2010time quantitative polymerase chain reaction, and diagnostic performance was assessed using receiver operating characteristic curve analysis.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n MicroRNA\u2010720 was significantly upregulated and microRNA\u20101275 significantly downregulated in NTD\u2010affected pregnancies compared with controls (\n p<\/jats:italic>\n \u2009<\u20090.001). Both microRNAs showed high diagnostic accuracy. Expression levels varied among NTD subtypes, with the highest microRNA\u2010720 levels observed in acrania, followed by encephalocele and spina bifida. MSAFP levels were also significantly higher in cases than in controls (\n p<\/jats:italic>\n \u2009<\u20090.001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Circulating microRNA\u2010720 and microRNA\u20101275 are promising noninvasive biomarkers that provide a molecular layer of validation for fetal NTDs. They may complement established tools by providing additional diagnostic confidence, particularly in cases with borderline biochemical profiles.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jog.70385","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T23:24:23Z","timestamp":1782775463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating\n microRNA<\/scp>\n \u2010720 and\n microRNA<\/scp>\n \u20101275 as Noninvasive Specific Test Compared With Maternal Serum Alpha\u2010Fetoprotein and Three\u2010Dimensional Ultrasound to Diagnose Fetal Neural Tube Defects"],"prefix":"10.1111","volume":"52","author":[{"given":"Haitham","family":"khaled","sequence":"first","affiliation":[{"name":"Prenatal Diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute National Research Centre Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Abd Almaguid","family":"Attia","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department, Faculty of Medicine Cairo University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona K.","family":"Farag","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute National Research Centre Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hala","family":"Abdelwahab","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department, Faculty of Medicine Cairo University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8933-989X","authenticated-orcid":false,"given":"Sameh M.","family":"Senousy","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute National Research Centre Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled R.","family":"Gaber","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute National Research Centre Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.13.2657"},{"key":"e_1_2_12_3_1","first-page":"290","article-title":"Sonographic Diagnosis of Neural Tube Defects at 12\u2009Weeks","volume":"359","author":"Buisson O.","year":"2002","journal-title":"Lancet"},{"key":"e_1_2_12_4_1","first-page":"673","article-title":"Late\u2010Term Abortion: Antecedent Conditions and Consequences to Women's Health","volume":"115","author":"Coleman P. 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