{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:41:43Z","timestamp":1792636903796,"version":"4.5.0"},"reference-count":82,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Despite studies highlighting mitochondrial dynamics in preeclampsia (PE), the regulatory mechanisms of mitochondrial dynamics\u2010related genes (MD\u2010RGs) in PE remain unclear. This study aims to explore these mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>PE\u2010related datasets (GSE10588 and GSE98224) and 23 MD\u2010RGs were used. Differential expression, protein\u2013protein interaction (PPI) network, machine learning (ML), gene expression, and receiver operating characteristic (ROC) analyses were conducted to screen for PE biomarkers. A nomogram was established to predict PE occurrence. Gene set enrichment analysis (GSEA) and immunoinfiltration analysis were subsequently performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Reverse transcription quantitative PCR (RT\u2013qPCR) was used to measure biomarker expression, identifying GLUL, GPX3, and KCND2 as PE biomarkers. GPX3 and KCND2 were upregulated, whereas GLUL was downregulated in PE. These biomarkers had area under the curve (AUC) values of 0.7\u20130.9 in the datasets, and the integrated nomogram reached 0.973, indicating precision in PE prediction. GSEA revealed that ribosome and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>E. coli<\/jats:italic>\n                      <\/jats:styled-content>\n                      coenriched KCND2 and GLUL. Immunoinfiltration analysis revealed that Tregs were significantly correlated with KCND2 and GLUL among differential immune cells in PE patients versus controls. Finally, expression validation revealed that GLUL was significantly upregulated and expressed in the control group, whereas GPX3 and KCND2 were highly expressed in PE patients, with KCND2 reaching significant levels, which is consistent with the bioinformatics analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In total, 3 mitochondrial dynamics\u2010related biomarkers (GLUL, GPX3, and KCND2) were identified, and the biomarker\u2010based nomogram was used to predict the onset risk of PE patients. The mining of these biomarkers contributed to an in\u2010depth understanding of the pathogenesis of MD\u2010RGs in PE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jog.70444","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:07:56Z","timestamp":1792454876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Validation of Biomarkers Associated With Mitochondrial Dynamics in Preeclampsia"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2668-471X","authenticated-orcid":false,"given":"Xueying","family":"Liu","sequence":"first","affiliation":[{"name":"Classics Department of Traditional Chinese Medicine Qingdao Traditional Chinese Medicine Hospital, Qingdao Hiser Hospital Affiliated of Qingdao University  Qingdao Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenru","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiao","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haining","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Affiliated Hospital of Shandong University of Traditional Chinese Medicine  Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-08937-3"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2020.101123"},{"key":"e_1_2_13_4_1","article-title":"ACOG Practice Bulletin No. 202: Gestational Hypertension and Preeclampsia","volume":"133","author":"Espinoza J. 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