{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:33:40Z","timestamp":1789853620330,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T00:00:00Z","timestamp":1376265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R01-GM57726"],"award-info":[{"award-number":["R01-GM57726"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R01-GM62508"],"award-info":[{"award-number":["R01-GM62508"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Wellcome Trust Research Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Intern Med"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>More than 500,000 hospitalized patients survive severe sepsis annually in the USA. Recent epidemiological evidence, however, demonstrated that these survivors have significant morbidity and mortality, with 3\u2010year fatality rates higher than 70%. To investigate the mechanisms underlying persistent functional impairment in sepsis survivors, here we developed a model to study severe sepsis survivors following cecal ligation and puncture (CLP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sepsis was induced in mice by CLP and survivors were followed for twelve weeks. Spleen and blood were collected and analyzed at different time points post\u2010sepsis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We observed that sepsis survivors developed significant splenomegaly. Analysis of the splenic cellular compartments revealed a major expansion of the inflammatory CD11b+ Ly\u20106CHigh pool. Serum high\u2010mobility group box 1 (HMGB1) levels in the sepsis surviving mice were significantly elevated for 4\u20106 weeks after post\u2010sepsis, and administration of an anti\u2010HMGB1 monoclonal antibody significantly attenuated splenomegaly as well as splenocyte priming. Administration of recombinant HMGB1 to naive mice induced similar splenomegaly, leukocytosis and splenocyte priming as observed in sepsis survivors. Interestingly analysis of circulating HMGB1 from sepsis survivors by mass spectroscopy demonstrated a stepwise increase of reduced form of HMGB1 (with known chemo\u2010attractant properties) during the first 3 weeks, followed by disulphide form (with known inflammatory properties) 4\u20108 weeks after CLP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Our results indicate that prolonged elevation of HMGB1 is a necessary and sufficient mediator of splenomegaly and splenocyte expansion, as well as splenocyte inflammatory priming in murine severe sepsis survivors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/joim.12104","type":"journal-article","created":{"date-parts":[[2013,6,28]],"date-time":"2013-06-28T04:14:56Z","timestamp":1372392896000},"page":"381-390","source":"Crossref","is-referenced-by-count":72,"title":["Expression of Concern:\n                    <scp>HMGB<\/scp>\n                    1 mediates splenomegaly and expansion of splenic\n                    <scp>CD<\/scp>\n                    11b+\n                    <scp>L<\/scp>\n                    y\u20106\n                    <scp>C<\/scp>\n                    <sup>high<\/sup>\n                    inflammatory monocytes in murine sepsis survivors"],"prefix":"10.1111","volume":"274","author":[{"given":"S. I.","family":"Vald\u00e9s\u2010Ferrer","sequence":"first","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"},{"name":"The Elmezzi Graduate School of Molecular Medicine Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rosas\u2010Ballina","sequence":"additional","affiliation":[{"name":"Focal Area Infection Biology Biozentrum, University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. S.","family":"Olofsson","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Lu","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"},{"name":"The Elmezzi Graduate School of Molecular Medicine Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"Dancho","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ochani","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. H.","family":"Li","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Scheinerman","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"Katz","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. A.","family":"Levine","sequence":"additional","affiliation":[{"name":"SetPoint Medical, Valen Inc. Valencia CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. K.","family":"Hudson","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yang","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. A.","family":"Pavlov","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Roth","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Blanc","sequence":"additional","affiliation":[{"name":"The Laboratory of Biomedical Sciences, The Feinstein Institute for Medical Research Manhasset NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. J.","family":"Antoine","sequence":"additional","affiliation":[{"name":"MRC Centre for Drug Safety Science, Molecular and Clinical Pharmacology University of Liverpool Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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