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During the first week, the single\u2010cell zygote undergoes eight to nine rounds of cell division generating the multicellular blastocyst, which consists of hundreds of cells forming spatially organized embryonic and extra\u2010embryonic tissues. At the level of transcription, degradation of maternal <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> commences at around the two\u2010cell stage, coinciding with embryonic genome activation. Although numerous efforts have recently focused on delineating this process in humans, many questions still remain as thorough investigation has been limited by ethical issues, scarce availability of human embryos and the presence of minute amounts of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. <jats:italic>In vitro<\/jats:italic> cultures of embryonic stem cells provide some insight into early human development, but such studies have been confounded by analysis on a population level failing to appreciate cellular heterogeneity. Recent technical developments in single\u2010cell <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> sequencing have provided a novel and powerful tool to explore the early human embryo in a systematic manner. In this review, we will discuss the advantages and disadvantages of the techniques utilized to specifically investigate human development and consider how the technology has yielded new insights into pre\u2010implantation development, embryonic stem cells and the establishment of the germ line.<\/jats:p>","DOI":"10.1111\/joim.12493","type":"journal-article","created":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T05:59:41Z","timestamp":1459835981000},"page":"252-264","source":"Crossref","is-referenced-by-count":19,"title":["Single\u2010cell <scp>RNA<\/scp> sequencing: revealing human pre\u2010implantation development, pluripotency and germline development"],"prefix":"10.1111","volume":"280","author":[{"given":"S.","family":"Petropoulos","sequence":"first","affiliation":[{"name":"Department of Clinical Science, Intervention and Technology Karolinska Institutet and Division of Obstetrics and Gynecology Karolinska University Hospital Stockholm Sweden"},{"name":"Ludwig Institute for Cancer Research Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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