{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T11:43:17Z","timestamp":1775043797974,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T00:00:00Z","timestamp":1505952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Intern Med"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Amiodarone is an effective and widely used antiarrhythmic drug with many possible adverse effects including hypercholesterolaemia and hepatotoxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Our aim was to evaluate how long\u2010term amiodarone treatment affects cholesterol metabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study population consisted of 56 cardiac patients, of whom 20 were on amiodarone (amiodarone + group) and 36 did not use the drug (amiodarone \u2212 group). We also studied a control group of 124 individuals selected randomly from the population. Cholesterol metabolism was evaluated by analysis of serum noncholesterol sterols by gas\u2013liquid chromatography and gas chromatography\u2013mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparisons of serum lipids and noncholesterol sterols across the three groups showed increased serum triglyceride in users of amiodarone but no statistically significant group differences in total, <jats:styled-content style=\"fixed-case\">LDL<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> cholesterol or serum proprotein convertase subtilisin\/kexin type 9 concentrations. Nor did the groups differ in the ratios of cholestanol or plant sterols to cholesterol in serum, suggesting that cholesterol absorption was unaltered. However, all users of amiodarone had very markedly elevated serum desmosterol concentrations: the desmosterol\u2010to\u2010cholesterol ratio (10<jats:sup>2<\/jats:sup> \u00d7 \u03bcmol mmol<jats:sup>\u22121<\/jats:sup>) averaged 1030.7 \u00b1 115.7 (mean \u00b1 <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content>) in the amiodarone + group versus 82.7 \u00b1 3.4 and 75.9 \u00b1 1.4 in the amiodarone \u2212 and the population control groups (<jats:italic>P<\/jats:italic> &lt; 0.001), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Use of amiodarone was associated with on average 12\u2010fold serum desmosterol concentrations compared with the control groups. This observation is fully novel and suggests that amiodarone interferes with the conversion of desmosterol to cholesterol in the cholesterol synthesis pathway. Whether accumulation of desmosterol plays a role in amiodarone\u2010induced hepatotoxicity deserves to be studied in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/joim.12682","type":"journal-article","created":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T08:52:42Z","timestamp":1504255962000},"page":"93-101","source":"Crossref","is-referenced-by-count":12,"title":["Desmosterol accumulation in users of amiodarone"],"prefix":"10.1111","volume":"283","author":[{"given":"P.","family":"Simonen","sequence":"first","affiliation":[{"name":"Heart and Lung Center, Cardiology Helsinki University Central Hospital University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Lehtonen","sequence":"additional","affiliation":[{"name":"Heart and Lung Center, Cardiology Helsinki University Central Hospital University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. \u2010M.","family":"Lampi","sequence":"additional","affiliation":[{"name":"Department of Food and Environmental Sciences University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Piironen","sequence":"additional","affiliation":[{"name":"Department of Food and Environmental Sciences University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U. \u2010H.","family":"Stenman","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Helsinki University Central Hospital University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kupari","sequence":"additional","affiliation":[{"name":"Heart and Lung Center, Cardiology Helsinki University Central Hospital University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4586-8367","authenticated-orcid":false,"given":"H.","family":"Gylling","sequence":"additional","affiliation":[{"name":"Internal Medicine Helsinki University Central Hospital University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.298.11.1312"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(88)90655-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1004928"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315226"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-114-2-128"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bcr.08.2011.4620"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(98)90277-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2015.08.022"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.07.090"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623311432290"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.05.037"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.04.004"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26342"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(69)90007-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38603-X"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(89)90252-7"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a115479"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39911-9"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2007.08.003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.112.969261"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.110.959254"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2016.03.018"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38566-7"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.150.3692.19"},{"key":"e_1_2_8_26_1","first-page":"2256","article-title":"Preputial gland tumor sterols. III. A metabolic pathway from lanosterol to cholesterol","volume":"235","author":"Kandutsch AA","year":"1960","journal-title":"J Biol Chem"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2013.09.002"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R113.479808"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.07999"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104323"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)65020-9"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1086\/323473"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.b.10873"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.34040"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2011.74"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-9-94"},{"key":"e_1_2_8_37_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0022-2275(20)39637-1","article-title":"Tissue distribution of cholesterol and 24\u2010dehydrocholesterol during chronic triparanol therapy","volume":"6","author":"Chobanian AV","year":"1965","journal-title":"J Lipid Res"},{"key":"e_1_2_8_38_1","first-page":"49","article-title":"Triparanol\u2010induced cataract in rats","volume":"61","author":"Sallmann L","year":"1963","journal-title":"Tr Am Opthalmol"}],"container-title":["Journal of Internal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjoim.12682","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joim.12682","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T20:49:03Z","timestamp":1695502143000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joim.12682"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,21]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1111\/joim.12682"],"URL":"https:\/\/doi.org\/10.1111\/joim.12682","archive":["Portico"],"relation":{},"ISSN":["0954-6820","1365-2796"],"issn-type":[{"value":"0954-6820","type":"print"},{"value":"1365-2796","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,21]]}}}