{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:18:22Z","timestamp":1787563102607,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Intern Med"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Recent genomic studies revealed enhancer of zeste homolog 2 (\n                      <jats:italic>EZH2<\/jats:italic>\n                      ) gain\u2010of\u2010function mutations, representing novel therapeutic targets in follicular lymphoma (FL) in around one quarter of patients. However, these analyses relied on single\u2010site tissue biopsies and did not investigate the spatial heterogeneity and temporal dynamics of these alterations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      We aimed to perform a systematic analysis of\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutations using paired tissue (tumor biopsies [TB]) and liquid biopsies (LB) collected prior to treatment within the framework of a nationwide multicentric study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Pretreatment LB and TB samples were collected from 123 patients. Among these, 114 had paired TB and LB, with 39 patients characterized with paired diagnostic and relapse samples available. The\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutation status and allele burden were assessed using an in\u2010house\u2010designed, highly sensitive multiplex droplet digital PCR assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutation frequency was found to be 41.5% in the entire cohort. In patients with paired TB and LB samples,\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutations were identified in 37.8% of the patients with mutations exclusively found in 5.3% and 7.9% of TB and LB samples, respectively.\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutation status switch was documented in 35.9% of the patients with paired diagnostic and relapse samples. We also found that\n                      <jats:italic>EZH2<\/jats:italic>\n                      wild\u2010type clones may infiltrate the bone marrow more frequently compared to the\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutant ones.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The in\u2010depth spatio\u2010temporal analysis identified\n                      <jats:italic>EZH2<\/jats:italic>\n                      mutations in a considerably higher proportion of patients than previously reported. This expands the subset of FL patients who most likely would benefit from EZH2 inhibitor therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/joim.13674","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T02:44:43Z","timestamp":1685587483000},"page":"295-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Parallel testing of liquid biopsy (ctDNA) and tissue biopsy samples reveals a higher frequency of\n                    <i>EZH2<\/i>\n                    mutations in follicular lymphoma"],"prefix":"10.1111","volume":"294","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6118-0365","authenticated-orcid":false,"given":"\u00c1kos","family":"Nagy","sequence":"first","affiliation":[{"name":"HCEMM\u2010SE Molecular Oncohematology Research Group, Department of Pathology and Experimental Cancer Research Semmelweis University  Budapest 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