{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:31:48Z","timestamp":1785198708086,"version":"3.55.0"},"reference-count":71,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Intern Med"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ferroptosis plays a key role in placental development and physiology, and abnormal ferroptosis has been implicated in trophoblast injury leading to preeclampsia (PE). We hypothesize that leukocytes isolated from PE exhibit increased ferroptosis and that extracellular vesicles contain long non\u2010coding (lnc) RNA\/mRNAs that modulate oxidative stress and iron toxicity in vascular endothelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We measured the expression of key regulators of ferroptosis in leukocytes and extracellular vesicles as well as circulating biomarkers of iron homeostasis and oxidative stress in plasma from women with\/without PE at different timepoints during pregnancy. For markers that were dysregulated, we assessed their temporal correlation with established markers of disease activity and marker of endothelial activation. For markers dysregulated in early pregnancy, we assessed their ability to predict the development of PE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found decreased lncRNA\/mRNAs in leukocytes, but not extracellular vesicles, in PE that may modulate oxidative stress and iron toxicity. This decrease in anti\u2010ferroptotic markers does not appear to be related to maternal disease activity or plasma oxidative stress status but rather to attenuated anti\u2010inflammatory expression in these cells. Circulating ferritin was elevated in PE, supporting the hypothesis that PE represents a disbalance in iron homeostasis. Low lncRNA taurine upregulated gene 1 RNA levels in leukocytes at 22\u201324 weeks were strongly associated with the development of PE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that maternal leukocytes in PE show decreased anti\u2010ferroptotic activity that correlates with anti\u2010inflammatory expression. Moreover, some of these changes in ferroptotic activity appear to precede the development of PE.<\/jats:p><\/jats:sec>","DOI":"10.1111\/joim.13732","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T16:13:58Z","timestamp":1698077638000},"page":"181-195","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Increased ferroptosis in leukocytes from preeclamptic women involving the long non\u2010coding taurine upregulated gene 1 (TUG1)"],"prefix":"10.1111","volume":"295","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7962-4726","authenticated-orcid":false,"given":"Tove","family":"Lekva","sequence":"first","affiliation":[{"name":"Research Institute of Internal Medicine Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika Elisabet","family":"Michelsen","sequence":"additional","affiliation":[{"name":"Research Institute of 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