{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T20:15:21Z","timestamp":1780085721180,"version":"3.54.0"},"reference-count":25,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,10,4]],"date-time":"2016-10-04T00:00:00Z","timestamp":1475539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["26293436"],"award-info":[{"award-number":["26293436"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["25713063"],"award-info":[{"award-number":["25713063"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study aimed to examine hyaluronan (<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>) metabolism in relation to the onset and progression of temporomandibular joint osteoarthritis (<jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>) induced by mechanical overloading. Two\u2010month\u2010old and 6\u2010month\u2010old C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6N mice were divided into experimental and untreated control groups (<jats:italic>n<\/jats:italic>= 5\/group). A sliding plate was attached to the maxillary incisors of the experimental mice for 10 days to overload the condylar cartilage in<jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content>. In experimental group, profound cartilage degradation was detected in haematoxylin\u2013eosin, Safranin\u2010O\u2010Fast Green\u2010stained sections. It was also shown that the cartilage degradation was greater in older mice in both the control and the experimental groups. The number of<jats:styled-content style=\"fixed-case\">HABP<\/jats:styled-content>\u2010positive cells was decreased by mechanical overloading and with age. The reduction of<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>expression was correlated with the progression of cartilage degradation induced by mechanical overloading. The absolute quantification of the<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression related to<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>synthesis and<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>degradation was also performed in each group. The<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression levels of<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>synthase (<jats:styled-content style=\"fixed-case\">HAS<\/jats:styled-content>) 2 and 3 were lower in the experimental group compared with the control group in the younger mice. In contrast, the<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression levels of the<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>degradation gene,<jats:styled-content style=\"fixed-case\">HYAL<\/jats:styled-content>2 and<jats:styled-content style=\"fixed-case\">KIAA<\/jats:styled-content>1199, were higher in the experimental group compared with the control group in the older mice. Thus, mechanical overload differently affected the balance of<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>degradation and<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>synthesis in the older and younger mice, respectively. In conclusion, mechanical overloading affects<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>metabolism and it might initiate or amplify the condylar cartilage degradation.<\/jats:p>","DOI":"10.1111\/joor.12443","type":"journal-article","created":{"date-parts":[[2016,9,14]],"date-time":"2016-09-14T15:16:38Z","timestamp":1473866198000},"page":"921-928","source":"Crossref","is-referenced-by-count":11,"title":["Hyaluronan metabolism in overloaded temporomandibular joint"],"prefix":"10.1111","volume":"43","author":[{"given":"T.","family":"Shinohara","sequence":"first","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Izawa","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics Institute of Biomedical Sciences Tokushima University 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