{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T04:19:57Z","timestamp":1773375597621,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T00:00:00Z","timestamp":1557532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81530033"],"award-info":[{"award-number":["81530033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500896"],"award-info":[{"award-number":["81500896"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Biomarkers of temporomandibular joint (TMJ) osteoarthritis (OA) remain unknown. The objective\u00a0was to\u00a0detect whether molecular biomarkers from peripheral blood leucocytes (PBLs) engage in TMJ OA lesions. Thirty\u2010four six\u2010week\u2010old Sprague Dawley rats were used. The top upregulated gene ontology categories and gene\u2010fold changes in PBLs were detected by a microarray analysis comparing rats that received 20\u2010week unilateral anterior crossbite (UAC) treatment with age\u2010matched controls (n\u00a0=\u00a04). Twenty weeks of UAC treatment had been reported to induce TMJ OA\u2010like lesions. The other twenty\u2010four rats were randomly placed in the UAC and control groups at 12\u2010 and 20\u2010week time points (n\u00a0=\u00a06). The mRNA expression levels of the selected biomarkers derived from the microarray analysis and their protein expression in the alveolar bone and TMJ were detected. The microarray analysis indicated that the three most highly involved genes in PBLs were <jats:italic>Egr1<\/jats:italic>, <jats:italic>Ephx1<\/jats:italic> and<jats:italic> Il10<\/jats:italic>, which were confirmed by real\u2010time PCR detection. The increased protein expression levels of the three detected molecules were demonstrated in cartilage and subchondral bone (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), and increased levels of EPHX1 were reported in discs (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05); however, increased levels were not present in the alveolar bone. Immunohistochemistry revealed the increased distribution of EGR1\u2010positive, EXPH1\u2010positive and IL10\u2010positive cells predominantly in the osteochondral interface, with EXPH1 also present in TMJ discs. In conclusion, the\u00a0increased mRNA expression of <jats:italic>Egr1, Ephx1<\/jats:italic> and<jats:italic> Il10<\/jats:italic> in PBLs may serve as potential biomarkers for developed osteoarthritic lesions relating to osteochondral interface hardness changes induced by dental biomechanical stimulation.<\/jats:p>","DOI":"10.1111\/joor.12810","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T13:14:56Z","timestamp":1556802896000},"page":"820-827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular changes in peripheral blood involving osteoarthritic joint remodelling"],"prefix":"10.1111","volume":"46","author":[{"given":"Hong\u2010Yun","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Military Stomatology, National Clinical Research Centre for Oral Disease &amp; Shaanxi International Joint Research Centre for Oral Diseases Department of 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The Jiamusi University Jiamusi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Burns and Cutaneous Surgery, Xijing Hospital The Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Xu","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology, National Clinical Research Centre for Oral Disease &amp; Shaanxi International Joint Research Centre for Oral Diseases Department of Oral Anatomy and Physiology and TMD, School of Stomatology The Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology, National Clinical Research Centre for Oral Disease &amp; Shaanxi International Joint Research 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