{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:39:26Z","timestamp":1761676766596,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Craniosynostosis, a severe craniofacial developmental disease, can only be treated with surgery currently. Recent studies have shown that proteoglycans are involved in the suture development. For the bone matrix protein, dentin matrix protein 1 (DMP1), glycosylation on the N\u2010terminal of it could generate a functional proteoglycan form of DMP1 during osteogenesis. We identified that the proteoglycan form of DMP1 (DMP1\u2010PG) is highly expressed in mineralisation front of suture. But, the potential role of DMP1\u2010PG in suture fusion remain unclear. To investigate the role of DMP1\u2010PG in cranial suture fusion and craniofacial bone development. By using a DMP1 glycosylation site mutation mouse model, DMP1\u2010S89G mice, we compared the suture development in it with control mice. We compared the suture phenotypes, bone formation rate, expression levels of bone formation markers in vivo between DMP1\u2010S89G mice and wild\u2010type mice. Meanwhile, cell culture and organ culture were performed to detect the differences in cell differentiation and suture fusion in vitro. Finally, chondroitin sulphate (CHS), as functional component of DMP1\u2010PG, was employed to test whether it could delay the premature suture fusion and the abnormal differentiation of bone mesenchymal stem cells (BMSCs) of DMP1\u2010PG mice. DMP1\u2010S89G mice had premature closure of suture and shorter skull size. Lack of DMP1\u2010PG accelerated bone formation in cranial suture. DMP1\u2010PG maintained the essential stemness of BMSCs in suture through blocking the premature differentiation of BMSCs to osteoblasts. Finally, chondroitin sulphate, a major component of DMP1\u2010PG, successfully delayed the premature suture fusion by organ culture of skull in vitro. DMP1\u2010PG could inhibit premature fusion of cranial suture and maintain the suture through regulating the osteogenic differentiation of BMSCs.<\/jats:p>","DOI":"10.1111\/joor.12881","type":"journal-article","created":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T22:41:01Z","timestamp":1567032061000},"page":"19-28","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Glycosylation of DMP1 maintains cranial sutures in mice"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6165-1913","authenticated-orcid":false,"given":"Mingxiang","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Oral Implantology School of Stomatology Shanghai Engineering Research Center of Tooth Restoration and Regeneration Tongji University Shanghai 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