{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:06Z","timestamp":1772160726869,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019\u2010ZD\u20100923"],"award-info":[{"award-number":["2019\u2010ZD\u20100923"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dental pulp tissues are rich in pain\u2010related afferent nerve fibers, which originate from primary sensory neurons in the trigeminal ganglion (TG). The mechanisms of central nervous system (CNS) underlying ectopic pain following peripheral inflammation have been reported that the macrophages as inflammatory and immunologic mediators in the TG play an important role in the process of pulpitis and hyperalgesia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective(s)<\/jats:title>\n                    <jats:p>To observe the polarization response and dynamic distribution of macrophages in the TG during the development of dental pulp inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rat model of pulpitis was established using complete Freund's adjuvant (CFA). Hematoxylin\u2010eosin (HE), immunohistochemistry (IHC), immunofluorescence (IF), toluidine blue (TB) staining, and RT\u2010qPCR were performed to observe the expression of macrophage\u2010related factors in the TG.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results of IHC staining showed that M2 macrophages labeled with CD206 were observed in the TG of both the control and CFA groups. The statistical analysis indicated that the number of CD206\u2010positive macrophages in the TG increased significantly at 24\u00a0h after CFA\u2010induced pulpitis, reached a peak at 2\u00a0weeks, and then returned to the normal level after 6\u00a0weeks. The ratio of M2\/M1 in the CFA groups was significantly lower than that in the control group from 24 to 72\u00a0h, and this pattern was reversed at 2\u00a0weeks after CFA\u2010induced pulpitis; then, the ratio increased significantly and was maintained at a high level for 4\u00a0weeks. RT\u2010qPCR results showed that the expression of IL\u201010 in the TG increased significantly from 1 to 4\u00a0weeks after CFA\u2010induced pulpitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The trend of M2 macrophages was opposite to that of M1 macrophages in the TG during the process of pulpitis induced by CFA, which is consistent with the expression of macrophage\u2010related cytokines. Macrophage polarization in the TG may participate in the neuroinflammation response induced by dental pulpitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/joor.13245","type":"journal-article","created":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T11:43:54Z","timestamp":1629114234000},"page":"228-236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Polarization of macrophages in the trigeminal ganglion of rats with pulpitis"],"prefix":"10.1111","volume":"49","author":[{"given":"Lu","family":"Gao","sequence":"first","affiliation":[{"name":"School of Stomatology Dalian Medical University Dalian China"},{"name":"The Affiliated Stomatological Hospital of Dalian Medical University Dalian China"},{"name":"Academician Laboratory of Immune and Oral Development &amp; Regeneration Dalian Medical University Dalian 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