{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T03:58:50Z","timestamp":1776830330895,"version":"3.51.2"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171303"],"award-info":[{"award-number":["32171303"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mesenchymal stem cells (MSCs) derived from the synovium, known as synovium mesenchymal stem cells (SMSCs), exhibit significant potential for articular cartilage regeneration owing to their capacity for chondrogenic differentiation. However, the microRNAs (miRNAs) governing this process and the associated mechanisms remain unclear. While mechanical stress positively influences chondrogenesis in MSCs, the miRNA\u2010mediated response of SMSCs to mechanical stimuli is not well understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study explores the miRNA\u2010driven mechano\u2010transduction in SMSCs chondrogenesis under mechanical stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The surface phenotype of SMSCs was analysed by flow cytometry. Chondrogenesis capacities of SMSCs were examined by Alcian blue staining. High throughput sequencing was used to screen mechano\u2010sensitive miRNAs of SMSCs. The RNA expression level of COL2A1, ACAN, SOX9, BMPR2 and miR\u2010143\u20103p of SMSCs were tested by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR). The interaction between miR\u2010143\u20103p and TLR4 was confirmed by luciferase reporter assays. The protein expression levels of related genes were assessed by western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>High\u2010throughput sequencing revealed a notable reduction in miR\u2010143\u20103p levels in mechanically stressed SMSCs. Gain\u2010 or loss\u2010of\u2010function strategies introduced by lentivirus demonstrated that miR\u2010143\u20103p overexpression hindered chondrogenic differentiation, whereas its knockdown promoted this process. Bioinformatics scrutiny and luciferase reporter assays pinpointed a potential binding site for miR\u2010143\u20103p within the 3\u2032\u2010UTR of bone morphogenetic protein receptor type 2 (BMPR2). MiR\u2010143\u20103p overexpression decreased BMPR2 expression and phosphorylated Smad1, 5 and 8 levels, while its inhibition activated BMPR2\u2010Smad pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study elucidated that miR\u2010143\u20103p negatively regulates SMSCs chondrogenic differentiation through the BMPR2\u2010Smad pathway under mechanical tensile stress. The direct targeting of BMPR2 by miR\u2010143\u20103p established a novel dimension to our understanding of mechano\u2010transduction mechanism during SMSC chondrogenesis. This understanding is crucial for advancing strategies in articular cartilage regeneration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/joor.13726","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T07:54:49Z","timestamp":1715154889000},"page":"1507-1520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>MiR<\/scp>\n                    \u2010143\u20103p regulates chondrogenic differentiation of synovium derived mesenchymal stem cells under mechanical stress through the\n                    <scp>BMPR2\u2010Smad<\/scp>\n                    signalling pathway by targeting\n                    <scp>BMPR2<\/scp>"],"prefix":"10.1111","volume":"51","author":[{"given":"Xiao","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5303-4528","authenticated-orcid":false,"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengmeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Qingdao Hospital University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital)  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2431-3488","authenticated-orcid":false,"given":"Zijing","family":"He","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dapeng","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration and Shandong Provincial Clinical Research Center for Oral Diseases  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3590-1805","authenticated-orcid":false,"given":"Xiao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1043\/0003-3219(2003)073<0631:GMOTRM>2.0.CO;2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0889-5406(89)90055-3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0889-5406(03)00149-5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11282-019-00379-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2009.06.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2319\/021710-99.1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/22.6.649"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(94)90191-0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516668321"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2003.10.002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3210"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2011.09.005"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5979741"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21212"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0308-z"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2008.11.017"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/454021"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9696378"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1121158"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.119"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201812_16649"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12688"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12794"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-019-01080-2"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2462-2"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2022.2099191"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977190412331279890"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3891"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.181"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-019-03538-3"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.01.015"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-022-03053-0"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101896"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045517"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.12.009"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2020.155352"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/15257770.2022.2057535"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.55664"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2018.6959"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020398"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29896"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.7518\/hxkq.2017.05.015"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2008.0130"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2014.2051"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2416-8"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2015.2275"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.05.049"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.7.3479"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2005.01.009"}],"container-title":["Journal of Oral Rehabilitation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joor.13726","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T13:13:24Z","timestamp":1752844404000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joor.13726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,8]]},"references-count":49,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/joor.13726"],"URL":"https:\/\/doi.org\/10.1111\/joor.13726","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JOOR.13726\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOOR.13726\/v3\/review1","asserted-by":"object"}]},"ISSN":["0305-182X","1365-2842"],"issn-type":[{"value":"0305-182X","type":"print"},{"value":"1365-2842","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,8]]},"assertion":[{"value":"2023-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}